Protein Info for AZOBR_RS17450 in Azospirillum brasilense Sp245
Annotation: putative SIMILAR TO BETA SUBuniT OF CITRATE LYASE protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 45% identity to sno:Snov_4286)Predicted SEED Role
No annotation
MetaCyc Pathways
- citrate degradation (2/2 steps found)
- reductive TCA cycle II (7/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.34, 4.1.3.6
Use Curated BLAST to search for 4.1.3.34 or 4.1.3.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8AS22 at UniProt or InterPro
Protein Sequence (287 amino acids)
>AZOBR_RS17450 putative SIMILAR TO BETA SUBuniT OF CITRATE LYASE protein (Azospirillum brasilense Sp245) MSLPYQSYLFVPADNAKLLEKAHQRGADALILDLEDAVLPAGKPEARRGLPEPIDRLHGL GVPVLVRINSGWRDAVADLEAAVRPGVAALVVPKAEDAGALRVLSAMIAEWEAERGLTPG AIGLVALIESPLGLERLSGIAAVPRVAALALGSEDFALTLGVEPTEALLALPCRQIALAA AARGLAAIGLPGSLAEFRDLDAYRAMVAQARAVGMTGALCIHPAQLPVVRDVFSPSAADV AWAGRVVAAWDEAQAAGRGAVQVDGRMVDRPVAERAKAILARRAPTA