Protein Info for AZOBR_RS17000 in Azospirillum brasilense Sp245

Annotation: 1-deoxy-D-xylulose-5-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13292: DXP_synthase_N
  • amino acids 13 to 287 (275 residues), 388.2 bits, E=3e-120
• TIGR00204: 1-deoxy-D-xylulose-5-phosphate synthase
  • amino acids 16 to 629 (614 residues), 771.3 bits, E=3.6e-236
• PF02779: Transket_pyr
  • amino acids 324 to 485 (162 residues), 147.2 bits, E=6e-47
• PF02780: Transketolase_C
  • amino acids 501 to 624 (124 residues), 92.3 bits, E=3.3e-30

Best Hits

Swiss-Prot: 78% identical to DXS_RHOCS: 1-deoxy-D-xylulose-5-phosphate synthase (dxs) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K01662, 1-deoxy-D-xylulose-5-phosphate synthase [EC: 2.2.1.7] (inferred from 86% identity to azl:AZL_c00990)

MetaCyc: 61% identical to 1-deoxy-D-xylulose 5-phosphate synthase (Arabidopsis thaliana col)
1-deoxy-D-xylulose-5-phosphate synthase. [EC: 2.2.1.7]

Predicted SEED Role

"1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)" in subsystem Isoprenoid Biosynthesis or Pyridoxin (Vitamin B6) Biosynthesis or Thiamin biosynthesis (EC 2.2.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (10/12 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (9/12 steps found)
• pyridoxal 5'-phosphate biosynthesis I (5/7 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
• taxadiene biosynthesis (engineered) (9/13 steps found)
• methylerythritol phosphate pathway I (6/9 steps found)
• methylerythritol phosphate pathway II (6/9 steps found)
• isoprene biosynthesis I (6/10 steps found)
• superpathway of ergosterol biosynthesis II (10/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.7

Use Curated BLAST to search for 2.2.1.7

Sequence Analysis Tools

See G8ARS3 at UniProt or InterPro

Protein Sequence (643 amino acids)

>AZOBR_RS17000 1-deoxy-D-xylulose-5-phosphate synthase (Azospirillum brasilense Sp245)
MKSKTPSSRTPLLDRVRTPVDLRRLKPRQLRQLADELRTETISAVSVTGGHLGAGLGVVE
LTVALHYVFDTPADRLIWDVGHQCYPHKILTGRRDRIRTLRTGGGLSGFTRRTESVYDPF
GAAHSSTAMSAGLGMAVARDRLGRRNHVVAVVGDGAMSAGMAYEAMNNIGSGDSRVIVVL
NDNNMSIAPPVGALSAYLSRLISSKPFLTLRHFAEDLAGHMPRPLRTAARRAEEYARGMV
TGGTLFEEMGFYYIGPIDGHNLDHLLPVLQNVRDAGDDKPVLIHVVTKKGKGYGPAEASA
DKLHAVAKFDVVTGAQSKPKSNAPTYTRVFANALIAEAERDPGVLAITAAMPSGTGLDLF
GQRFPDRCFDVGIAEQHAVTFAAGLATEGFKPFCAIYSTFLQRAYDQVVHDVVLQRLPVR
FALDRAGLVGADGATHAGAFDVAYLGCLPDIVLMAAADELELMHMVATSAAIDDRVSALR
YPRGEGVGLELPERGEVLPIGKGRILQEGTKVAILSYGTRLAEARKAAAELGARGLSTTV
ADARFAKPLDEDLVRRLALEHEVLITIEEGSVGGFGSFVLQHLAMAGLLDGGLKIRPMVL
PDRFLDHDSPARQYEEAGLAARHIVATALQALGNHSGRIATLG