Protein Info for AZOBR_RS16740 in Azospirillum brasilense Sp245

Annotation: cob(II)yrinic acid a c-diamide reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 TIGR02476: 5,6-dimethylbenzimidazole synthase" amino acids 6 to 210 (205 residues), 292 bits, see alignment E=9.9e-92 PF00881: Nitroreductase" amino acids 19 to 185 (167 residues), 117.6 bits, see alignment E=3.5e-38

Best Hits

Swiss-Prot: 60% identical to BLUB_RHIME: 5,6-dimethylbenzimidazole synthase (bluB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K04719, 5,6-dimethylbenzimidazole synthase [EC: 1.14.99.40] (inferred from 83% identity to azl:AZL_d03400)

MetaCyc: 60% identical to aerobic 5,6-dimethylbenzimidazole synthase (Sinorhizobium meliloti)
RXN-8771 [EC: 1.13.11.79]

Predicted SEED Role

"Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.79 or 1.14.99.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ARL7 at UniProt or InterPro

Protein Sequence (219 amino acids)

>AZOBR_RS16740 cob(II)yrinic acid a c-diamide reductase (Azospirillum brasilense Sp245)
MSDHAFAPEERDALYKAIFSRRDVRGQFAPDPVPEEVLTRVLTAAHHAPSVGFMQPWNFI
VVQDPAVKAQIHADFSQANEEEAALFTGERKALYSRLKLEGIREAPVNLCITCDRERSGP
VVLGRTHIPTMDLYSCVCAVQNLWLAARAEGLGVGWVSILHEDRLREALGIPERIVPVAY
LCLGYVSHFHETPELEKAGWRNRLPLEDLVFRDRWGGTT