Protein Info for AZOBR_RS16615 in Azospirillum brasilense Sp245

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 914 TIGR02401: malto-oligosyltrehalose synthase" amino acids 2 to 912 (911 residues), 1002.4 bits, see alignment E=9.5e-306 PF00128: Alpha-amylase" amino acids 24 to 92 (69 residues), 53 bits, see alignment 2.1e-18

Best Hits

KEGG orthology group: K06044, (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase [EC: 5.4.99.15] (inferred from 76% identity to azl:AZL_a05610)

Predicted SEED Role

"Malto-oligosyltrehalose synthase (EC 5.4.99.15)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 5.4.99.15)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ARI9 at UniProt or InterPro

Protein Sequence (914 amino acids)

>AZOBR_RS16615 alpha-amylase (Azospirillum brasilense Sp245)
IPLATYRVQLRAEFGFDRTAGIADYMARLGVSHLYASPYMKARPGSTHGYDIVDHNALNP
ELGSEESFHAMVEALKRNGLGQILDFVPNHMGVGGSDNGWWLDVLEWGPDSPYAGYFDIE
WEPDRRYLHGKVLVPLLGDQFGAVLESGGLELRFDREAGGFAVWAYDTHKLPIRPADYGT
ILGDDQPDLERIGDAFRHLDQVRPHQIRRANALKAELAALVSEKPDVAEAVERRLKRFRG
AEGEPESWRRLTDLIAAQNWRAAYFKVAADDINYRRFFNINELAGLRMEERELFDVAHRL
AFKLVDDGTLDGLRIDHIDGLYDPKGYCERLAQATERPFYLVVEKILARHERLREDWPID
GTTGYEFANLMGGLFVDPRGEEAFTKLYADFTGRRESFDEVVRTSKIRIMESEMASELHV
LARQASRIARANPRTADFTANILHQALKETIARFPVYRTYVDWRGVSELDRRSIDWAITQ
ARKADPNTDASAYDFLHRLLTTDLVAQPRSGYSRQQVLRFAMRFQQYSGPVMAKGLEDTA
FYRYNRLAALNEVGGHPEHFGVGSANFHNANAERAARWPHAMLGSTTHDTKRGEDTRARL
YALSEMPEEWERQIQTWSRLLRARRGDVEGTAPPDRNDEYLFYQLLVGAWPAELTGADPA
SLDPQAMTEFAERLAGAMTKSMREAKVHSTWAAPDEAYESAMTSFVHDALDVSRSTAFFD
AFLPFQERLARIGMVNGLTQTLLKLTSPGVPDIYQGCELWNFSLVDPDNRRPVDYDARRR
LLDEVEGAEIGSLLSRWQDGAVKLSLTRRALALRTAMPDLFAKGEYLPLEATGERAEHVV
AFARRLGDETVVVAAPRLVGALGETPEWGDTSVPLPRGQRWRNALTDGTLEAADAVTAAD
LFRDFPVALLVREG