Protein Info for AZOBR_RS16480 in Azospirillum brasilense Sp245

Annotation: hemolysin secretion protein D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 42 to 373 (332 residues), 264.5 bits, see alignment E=5.6e-83 PF16576: HlyD_D23" amino acids 59 to 291 (233 residues), 43.2 bits, see alignment E=4.3e-15 PF13533: Biotin_lipoyl_2" amino acids 61 to 105 (45 residues), 40.4 bits, see alignment 3e-14

Best Hits

KEGG orthology group: None (inferred from 53% identity to pzu:PHZ_c1077)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ARF7 at UniProt or InterPro

Protein Sequence (386 amino acids)

>AZOBR_RS16480 hemolysin secretion protein D (Azospirillum brasilense Sp245)
MLLAAIAALGGLWLAGPSGNRARAAETAQNAPPPVTVSPPLRKEIVEWDEFTGQFQAVDF
VEIRARVSGYLDSIAFQDGQLVKQGDPLFVIDPRPFEAALASARAEQQQAEAKLDLSNRQ
LARASELRRSDNVAASVFDERQQQVRVDAAGVEVAKAAVRTAELNLNFTRITAPIAGRIS
RREVSVGNLIVGGDTSTTTLLTTIVSLDPIYFIFDMSESDYLAYQRAAARGALKSQRDGG
IEVEGRLFDEQSWPLKGTLNFIDNQVSPGAGTIRARAVFPNPSLLLTPGQFGRLRLPGSD
RYTATLIPDQAVVSDQANKIVLTVGNDGTVVPKPVRLGPIEDGLRVVRSGLEETDKVIIN
GLVRARPGAKVTPQDGRIEPPKPPAG