Protein Info for AZOBR_RS15935 in Azospirillum brasilense Sp245

Annotation: tRNA-dihydrouridine synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 TIGR00742: tRNA dihydrouridine synthase A" amino acids 6 to 318 (313 residues), 466.3 bits, see alignment E=2.9e-144 PF01207: Dus" amino acids 8 to 304 (297 residues), 281.6 bits, see alignment E=3.9e-88

Best Hits

Swiss-Prot: 62% identical to DUSA_VIBCH: tRNA-dihydrouridine(20/20a) synthase (dusA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 88% identity to azl:AZL_009510)

MetaCyc: 62% identical to tRNA-dihydrouridine synthase A (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase A"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AR29 at UniProt or InterPro

Protein Sequence (329 amino acids)

>AZOBR_RS15935 tRNA-dihydrouridine synthase A (Azospirillum brasilense Sp245)
MPAIALSVAPMMDWTDRHCRTFHRLLSKNTLLYTEMVTTGAVLHGDRERLLGYDAAEHPV
ALQLGGSDPAELAACARIAEEWGYDEVNLNVGCPSDRVQSGRFGACLMAEPDLVARLVGA
MREAVSIPITVKSRIAIDEMEEWPTLDQFVRTVSAAGCTHFIVHARKAWLKGLSPKENRD
IPPLRYDLVHRLKQENPQLTIAINGGIRTLDEAEGHLATLDSVMIGRAAYETPYILADAD
RRLFGGEPGPDRYAVVEAMAAYAEERMRQGAPLSAITRHMLGLFQGLPGARAWRRHISEN
AHLPGSGPEVLLKAAALVPHREAVTQPTG