Protein Info for AZOBR_RS15320 in Azospirillum brasilense Sp245

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 945 TIGR01063: DNA gyrase, A subunit" amino acids 12 to 891 (880 residues), 1157.1 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 36 to 501 (466 residues), 545 bits, see alignment E=1.4e-167 PF03989: DNA_gyraseA_C" amino acids 533 to 580 (48 residues), 51.1 bits, see alignment 7.2e-18 amino acids 585 to 631 (47 residues), 18.7 bits, see alignment 9.3e-08 amino acids 643 to 688 (46 residues), 34.1 bits, see alignment 1.5e-12 amino acids 691 to 735 (45 residues), 41.5 bits, see alignment 7.1e-15 amino acids 793 to 840 (48 residues), 44.2 bits, see alignment 1e-15 amino acids 845 to 890 (46 residues), 40.5 bits, see alignment 1.4e-14

Best Hits

Swiss-Prot: 59% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 88% identity to azl:AZL_013920)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AQP0 at UniProt or InterPro

Protein Sequence (945 amino acids)

>AZOBR_RS15320 DNA gyrase subunit A (Azospirillum brasilense Sp245)
LSTTPLPPASDIAPVTIEDEMRRSYLDYAMSVIVSRALPDVRDGLKPVHRRILYAMKEGG
YDSTKPYKKSARIVGDVMGKYHPHGDSAIYDAMVRMAQDFSMRLPLIDGQGNFGSMDGDP
AAAMRYTEARLAKAAEALLDDIDKDTVDFQANYDDSGKEPTVVPARFPNLLVNGAGGIAV
GMATNIPTHNLSEVVDACCAYIDNNDVTDEELMEFVPGPDFPTGGMILGRSGVRSALMTG
RGSVIIRAKTEIEEIRKDRFAIVATEIPYQVNKSKLMERIGEVVNDKTIEGIADLRDESD
RDGVRVVIELKRDAVPDVVLAQLFRHTQLQTSFGVNMLALNGGRPELMNLRQIIAAFVRF
REQVITRRTEFLLGKARERAHTLVGLAVAVANLDAMIALIRSAPDPVWAREQMMEREWPA
MDVAPLIALIDEPGRGVSENGTYRLSEEQARAILDLRLHRLTGLERDRIGAELKDVTDQI
ADYLETLANRAKLLGILRDELVEMKERFGTPRRTEIQELEFEADIEDLIQREDMVVTVSQ
SGYVKRVPLSTYRAQKRGGKGRSGMSMKAEDAVSDLFVANTHTPLLLFSSRGMVYKLKVY
RLPLGNPQARGKAFVNLLPLIDGETITTVLPLPEDEAVWADLHVVFATSKGNVRRNRLSD
FANIRSNGLIAMKLEEEGERLIAVRTCSEADDVLLATGGGKCIRFEVADVRVFAGRTSTG
VRGIRLADGDEVISMTTLHHVESSPEERAAFLKRKREEGVDMEGEAAPEADEVSTESVTL
SPERYEELKGLEQYVLTVTERGYGKRTSSYEYRVTGRGGQGIWNMEMGERNGSIVAAFPV
EDSHQVMMVTNGGQVIRMPIHDVRIAGRKTLGVTLFRVGADERVVSVASIAEDEDTNGNG
ANGNGGDPNGGDPNGSDPNGGGTASVDDAAGNEAAPGDTPGDTIE