Protein Info for AZOBR_RS14655 in Azospirillum brasilense Sp245

Annotation: nodulation protein NolG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1048 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 338 to 356 (19 residues), see Phobius details amino acids 362 to 383 (22 residues), see Phobius details amino acids 389 to 411 (23 residues), see Phobius details amino acids 432 to 454 (23 residues), see Phobius details amino acids 460 to 482 (23 residues), see Phobius details amino acids 534 to 551 (18 residues), see Phobius details amino acids 860 to 877 (18 residues), see Phobius details amino acids 888 to 908 (21 residues), see Phobius details amino acids 914 to 937 (24 residues), see Phobius details amino acids 960 to 981 (22 residues), see Phobius details amino acids 993 to 1017 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 4 to 1014 (1011 residues), 931.2 bits, see alignment E=1.1e-283 PF03176: MMPL" amino acids 344 to 489 (146 residues), 21.6 bits, see alignment E=1.4e-08 amino acids 812 to 1018 (207 residues), 26.4 bits, see alignment E=5.2e-10 PF02355: SecD_SecF" amino acids 344 to 481 (138 residues), 20.8 bits, see alignment E=3.3e-08

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 84% identity to azl:AZL_011100)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AQ75 at UniProt or InterPro

Protein Sequence (1048 amino acids)

>AZOBR_RS14655 nodulation protein NolG (Azospirillum brasilense Sp245)
MPITRISVDNPVFATMMMVALMVLGLFSYNRLGVDQFPDVDFPLVVISTEYPGASPESVE
TDVTRPVEDAVNTIAGIKTLTSRSYESQSVVIAEFDLKTASTQALQDVREKVSALRPKFR
DEVKDPQITRFNPDDQPILSLAVKSDIRSLRDLTTMTDQIVLKRLQNVRGVGRATIAGGV
KRQVQVRLRPERLEALGVGVDQVLKAIRDENQDIPAGTVSGNGAERVVQVDGRVINPREL
LDIIVARRGGEPVRLRQVAEVLDGQEEQDSVALFNGQPALAVDVVKIQGSNTVEVARGLY
KALDELRRDGSLPSDVALEVVRDTSRGITNSLSNVQRTLVEGGVLTIAIVMIFLGSWRST
VITALTLPVAVMGTFGVLAAFGFTLNTMTLMALSLAIGILIDDAIVVRENIMRHLGKGQG
HRQAALDGTKEIGLAVLATTLTIVAVFLPVAFMGGIIGRFFLQFGITVSAAVLISLFVSF
TLDPMLSSLWYDPAAHGRHGRGVFGRFAAGFQGAFDALARVYGRTLRWALRWRWLVLLMA
LGIFVGSFFLVPRIGVEFVPAADLGESIVDVETPVGSSLDYTAAKLRQVEAAIREFPEVA
YTYSTVNTGASVGKNRGSVYVRLKPIDQRTRTPNTLAPPLRERLSAIPGVQIGIGIPGVG
GVQKQIQVSVQGRDIAELDRIGKQVTAAMAGIRGFVDVDVSLKAAKPTLSVRLERDLASD
LGVGTAQVANTLRPLFAGDKVSTWKAPDGETYDVLVRLPENDRVGRADLDRIYLTGSNDA
EGAPRMVPLSQVSRVETTLGASQINRRDLSREVNVQANVQGRPAGDAGKELQAAIAEIKL
PPGYRIVFGGSTKDIAETSAYATQALLLAVILIYLILASQFGSFLQPIAIMMSLPLSLVG
VFLGLLVAGSTLNIFSAIGFIMLMGLVTKNAILLVDFANQARARGVDLRDAVVEAGIIRL
RPIVMTTMAMIFGMIPLALGIGEGAQQRAPMAHAVIGGLISSTLLTLLVVPVILTYLDAL
SRRFKRWFAHKDPDAVAHGTAAQGTAAE