Protein Info for AZOBR_RS14425 in Azospirillum brasilense Sp245

Annotation: ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 PF00989: PAS" amino acids 34 to 136 (103 residues), 68.7 bits, see alignment E=1.1e-22 PF13188: PAS_8" amino acids 36 to 85 (50 residues), 29 bits, see alignment 1.9e-10 TIGR00229: PAS domain S-box protein" amino acids 36 to 107 (72 residues), 32.4 bits, see alignment E=4.5e-12 PF08448: PAS_4" amino acids 37 to 135 (99 residues), 46.7 bits, see alignment E=8.4e-16 PF00512: HisKA" amino acids 159 to 215 (57 residues), 47 bits, see alignment 5.6e-16 PF02518: HATPase_c" amino acids 261 to 379 (119 residues), 68.4 bits, see alignment E=1.8e-22

Best Hits

Swiss-Prot: 99% identical to NTRB_AZOBR: Sensory histidine kinase/phosphatase NtrB from Azospirillum brasilense

KEGG orthology group: K07708, two-component system, NtrC family, nitrogen regulation sensor histidine kinase GlnL [EC: 2.7.13.3] (inferred from 80% identity to azl:AZL_015080)

Predicted SEED Role

"Nitrogen regulation protein NtrB (EC 2.7.13.3)" (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AQ22 at UniProt or InterPro

Protein Sequence (400 amino acids)

>AZOBR_RS14425 ATPase (Azospirillum brasilense Sp245)
MARASAAAPLPRRPARPRAPSGSYRPVRSCIDPSVMLNALPDPVLVVDGSGDIRYVNLEA
QEFFGLSAAMMEGMPLAELLPPNSPVSQLIEQVQQGRHRASQEGVVIDTPRIGPHHVTVR
VTALGEPADHVLLTVNERTLARKIDNSLTHRNAARSVTAMASMLGHEVKNPLSGIRGAAQ
LLEENCSESDRVLTRLICDEADRIVALVNRMEVFSDQRPLERDAVNIHTVLEHVRKVAQS
GFARNIRFIERYDPSLPPVYGNRDQLIQIFLNLIKNAAEAAPESGGEIILSTSYQHGVRM
ALPGGDTRLHLPLLVSVQDNGDGIPDDLRSNLFDAFITTKVNGTGLGLALVAKIVGDHGG
VIEFDSQPRRTVFKVSLPMFDEAQMSGDPAPARGFRGAIG