Protein Info for AZOBR_RS14420 in Azospirillum brasilense Sp245

Annotation: tRNA-dihydrouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 TIGR00737: putative TIM-barrel protein, nifR3 family" amino acids 5 to 320 (316 residues), 403.8 bits, see alignment E=2.6e-125 PF01207: Dus" amino acids 15 to 318 (304 residues), 402.5 bits, see alignment E=5.9e-125

Best Hits

Swiss-Prot: 99% identical to DUS_AZOBR: Probable tRNA-dihydrouridine synthase (dus) from Azospirillum brasilense

KEGG orthology group: K05540, tRNA-dihydrouridine synthase B [EC: 1.-.-.-] (inferred from 82% identity to azl:AZL_015090)

Predicted SEED Role

"tRNA dihydrouridine synthase B (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AQ21 at UniProt or InterPro

Protein Sequence (328 amino acids)

>AZOBR_RS14420 tRNA-dihydrouridine synthase (Azospirillum brasilense Sp245)
MAIQIGTISLEGPVILAPMSGVTDLPFRRLVKQSGCGLVVSEMVASQAMIRENRQTLRMV
ECEPEQFPMAVQLAGCEPDVMAEAAKLNEDRGAAIIDINFGCPVKKVVNGHAGSSLMRDE
ALAARILEATAKAVSIPVTLKMRKGWDDNSLNAPRLARIAEECGIKLVTVHGRTREQFYN
GTADWSFIRSVKEAVSIPVVVNGDITSFDAVDRALAESGADGVMIGRGAYGRPWFPAQVM
HYIRTGERLPDPPLHEQLDTLLEHYDAMLTHYGVEGGLRVARKHISWYSTGLRDSAEFRS
EVNRMSDPERVRGFIRDFYAPAIERMAA