Protein Info for AZOBR_RS14390 in Azospirillum brasilense Sp245

Annotation: dihydrolipoamide dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 5 to 465 (461 residues), 596.4 bits, E=1.7e-183
• PF07992: Pyr_redox_2
  • amino acids 6 to 326 (321 residues), 236.2 bits, E=2.2e-73
• PF01134: GIDA
  • amino acids 7 to 146 (140 residues), 25.7 bits, E=2.4e-09
• PF12831: FAD_oxidored
  • amino acids 7 to 41 (35 residues), 32.2 bits, 3.1e-11
• PF00890: FAD_binding_2
  • amino acids 7 to 44 (38 residues), 28.3 bits, 4.4e-10
• PF13738: Pyr_redox_3
  • amino acids 9 to 310 (302 residues), 61.6 bits, E=3.1e-20
• PF00070: Pyr_redox
  • amino acids 177 to 252 (76 residues), 75.5 bits, E=1.6e-24
• PF02852: Pyr_redox_dim
  • amino acids 346 to 454 (109 residues), 130 bits, E=1.8e-41

Best Hits

Swiss-Prot: 48% identical to DLDH2_BACSU: Dihydrolipoyl dehydrogenase (bfmBC) from Bacillus subtilis (strain 168)

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 89% identity to azl:AZL_015170)

MetaCyc: 40% identical to dihydrolipoyl dehydrogenase monomer (Homo sapiens)
1.8.1.-; GCVMULTI-RXN [EC: 1.4.1.27]; 2-oxoisovalerate dehydrogenase (acylating). [EC: 1.4.1.27, 1.2.1.25]; 1.2.1.25 [EC: 1.4.1.27, 1.2.1.25]; 1.2.1.25 [EC: 1.4.1.27, 1.2.1.25]; 1.2.1.25 [EC: 1.4.1.27, 1.2.1.25]; 2OXOGLUTARATEDEH-RXN [EC: 1.4.1.27, 1.2.1.25, 1.2.1.105]; PYRUVDEH-RXN [EC: 1.4.1.27, 1.2.1.25, 1.2.1.105, 1.2.1.104]

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• anteiso-branched-chain fatty acid biosynthesis (31/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (31/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (31/34 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• TCA cycle III (animals) (9/10 steps found)
• L-leucine degradation I (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• L-valine degradation I (7/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• 2-oxobutanoate degradation I (4/4 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate V (3/3 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• L-isoleucine degradation I (5/6 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• L-threonine degradation V (2/2 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (2/3 steps found)
• glycine degradation (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• folate transformations II (plants) (7/11 steps found)
• photorespiration II (6/10 steps found)
• folate transformations I (8/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• L-lysine degradation II (L-pipecolate pathway) (4/9 steps found)
• L-lysine degradation XI (1/5 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• L-tryptophan degradation XI (mammalian, via kynurenine) (4/23 steps found)
• superpathway of L-lysine degradation (15/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.2.1.104 or 1.2.1.105 or 1.2.1.25 or 1.4.1.27 or 1.8.1.4

Sequence Analysis Tools

See G8AQ15 at UniProt or InterPro

Protein Sequence (465 amino acids)

>AZOBR_RS14390 dihydrolipoamide dehydrogenase (Azospirillum brasilense Sp245)
MADMNYDLIVIGGGPGGYVAAIRAAQLGLSTAVVERENLGGICLNWGCIPTKALLRSAEV
LRNAKHASEYGLVIQNPSFDLDKVVQRSRKVAGQLNGGVKHLLKKNKVAVIEGEAKLLGK
GQVAVTKGGAAVGTFGAKNIIIATGARARTLPGLEDDGNLVWTYRKAMTPNTTPKSLLVI
GSGAIGIEFASFYNELGAKVTVVEVMDRILPVEDEEISAFARKQFEKQGMRIITNGKAGN
LRKGADSVTVAVEANGKTEDITVDRVILAVGIAPNTENLGLENTKVQTDRGHIKTNANCQ
TDEPGVYAIGDVTGAPWLAHKASHEGVIAVEHIAGKHPHALDVRNIPGCTYSHPQIASVG
LTEKKAKEAGYEVRVGRFPFIGNGKAIALGEADGMVKTVFDAKTGELLGAHMVGAEVTEL
IQGYTVAKTMETTEQELMQTVFPHPTLSEMMHESVLDAYGRAIHF