Protein Info for AZOBR_RS13270 in Azospirillum brasilense Sp245

Annotation: phosphoketolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 810 PF09364: XFP_N" amino acids 12 to 366 (355 residues), 595 bits, see alignment E=7e-183 PF03894: XFP" amino acids 396 to 572 (177 residues), 297.8 bits, see alignment E=3.8e-93 PF09363: XFP_C" amino acids 583 to 784 (202 residues), 323.9 bits, see alignment E=5e-101

Best Hits

Swiss-Prot: 78% identical to PHK1_RHIME: Probable phosphoketolase 1 (R00106) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01632, fructose-6-phosphate phosphoketolase [EC: 4.1.2.22] (inferred from 76% identity to ava:Ava_0496)

Predicted SEED Role

"Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22)" (EC 4.1.2.22, EC 4.1.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.22 or 4.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AGT2 at UniProt or InterPro

Protein Sequence (810 amino acids)

>AZOBR_RS13270 phosphoketolase (Azospirillum brasilense Sp245)
MAEALSVKEKALIDAYWRAANYLSVGQIYLYDNPLLKVPLTLEHIKPRLLGHWGTTPGLN
FIYVHLNRMIRQLDLNMICIAGPGHGGPAMVANAYLEGTYSEVYPNISLDEEGIKRLFTQ
FSFPGGIPSHVAPETPGSIHEGGELGYAVSHAYGAAFDNPDLIVACIVGDGEAETGPLAT
SWHSNKFLNPLSDGAVLPILHLNGYKIANPTILSRISHEELEQLFRGYGYIPYFVEGHEP
DDMHERMASALDTAIADIRRIKDDARRNGVTQRPRWPLIVLRTPKGWTCPKEIDGKRAED
FWRSHQVPMGEMHENPDHVRVLEEWMKSYRPEELFDEGGRLRPDLADLAPRGIRRMSANP
HANGGTLLRDLRLPDFRDYAVPVEAPGVAVAEATRVMGRFLRDVMKPNLDARNFRLFSPD
ENNSNRWQDVLQVTDRTWVADTVPWDDHLAPDGRVMEMLSEHQCQGWLEGYLLTGRHGFF
SCYEAFIHIVDSMFNQHAKWLKVCRTIPWRRPVASLNYLLSSHVWRQDHNGFSHQDPGFI
DHVVNKKADVVRVYLPPDANCLLSVTDHCLRSRNCVNVVVAGKQPAPQWLTMDEAIRHCT
AGLGIWEWASNDRGGEPDVVMACCGDVPTLETLAAVDLLRHYAPDLKVRVINVVDLMTLQ
PPGEHPHGLPDRDFDALFTTDKPIIFAFHGYPWLIHRLTYRRAGHGNLHVRGYKEEGTTS
TPFDMCVLNDLDRFHLVGDVIDRVPALGASAAYVKQAMRDKLIAHKEHIRRYGDDMPEIA
GWIWDQTGAGQPGASRTGAVAAASTEADNV