Protein Info for AZOBR_RS12740 in Azospirillum brasilense Sp245

Annotation: dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 587 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 36 to 557 (522 residues), 807.2 bits, see alignment E=3.3e-247 PF13360: PQQ_2" amino acids 78 to 233 (156 residues), 64.6 bits, see alignment E=1.7e-21 amino acids 472 to 540 (69 residues), 36.2 bits, see alignment E=7.8e-13 PF13570: PQQ_3" amino acids 78 to 118 (41 residues), 20.3 bits, see alignment 9.2e-08 amino acids 481 to 521 (41 residues), 31 bits, see alignment 3.7e-11 PF01011: PQQ" amino acids 100 to 126 (27 residues), 25.3 bits, see alignment (E = 1.4e-09) amino acids 149 to 179 (31 residues), 26.2 bits, see alignment (E = 7.3e-10) amino acids 503 to 539 (37 residues), 39.3 bits, see alignment 5.1e-14

Best Hits

Swiss-Prot: 58% identical to EXAA_PSEAE: Quinoprotein alcohol dehydrogenase (cytochrome c) (exaA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 84% identity to azl:AZL_a05260)

MetaCyc: 57% identical to quinoprotein alcohol dehydrogenase (Pseudomonas putida U)
1.1.2.-

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AFY6 at UniProt or InterPro

Protein Sequence (587 amino acids)

>AZOBR_RS12740 dehydrogenase (Azospirillum brasilense Sp245)
MKRLFRTCLLASAMAAGLGLSGPALAAAPVAGGPGNDDLLNDATSTGDVLTYGMGPQAQR
FSPLDKLNPDTVKGLVPVWSFSFGGEKQRGQESQPLIHDGTIYVTGSYSRLYAVDVKTGQ
KKWQYDHRLPEGIMPCCDVVNRGAALYKDKIYFATLDARLVALNKDTGKVVWSKKIEDYK
AGYSATAAPLIVDGKVITGNSGGEFGIIGMVEARDAETGELVWQRPTIEGNMGTLNGKES
TMTGVMNASWSGDLYKTGGGATWLGGTYDPETKTLFFGTGNPAPWNSHLRPGDNLYTSST
LAIDPANGDIKWHYQTTPHDGWDFDGVNELVSFDLKKDGQTIKAGAKADRNGFFYVLDRT
NGKLLSASPFVTKITWAKEIDVKTGRPVYNDDNRPGTPVGAEKGTSVFAAPAFLGAKNWM
PMAYSPATELFYVPANEWGMDIWNEPITYKKGAAYLGAGFTIKPLYDDYIGALRAIDPKT
GKIVWEYKNPAPLWGGVLTTGGNLVFTGTPEGYLKAFDAKSGKEVWKFNTGSGVVGSPVT
WEQDGEQYVAVVSGWGGAVPLWGGDVAKLVKDINQGGSLWVFKLPKA