Protein Info for AZOBR_RS12500 in Azospirillum brasilense Sp245

Annotation: bifunctional enoyl-CoA hydratase/phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01575: MaoC_dehydratas
  • amino acids 32 to 131 (100 residues), 69.6 bits, E=2.8e-23
• PF13452: MaoC_dehydrat_N
  • amino acids 36 to 147 (112 residues), 23.8 bits, E=6.6e-09
• PF01515: PTA_PTB
  • amino acids 255 to 467 (213 residues), 148.2 bits, E=5.7e-47

Best Hits

KEGG orthology group: K00625, phosphate acetyltransferase [EC: 2.3.1.8] (inferred from 86% identity to azl:AZL_006980)

Predicted SEED Role

"Phosphate acetyltransferase (EC 2.3.1.8)" in subsystem Ethanolamine utilization or Fermentations: Lactate or Fermentations: Mixed acid or MLST or Propanediol utilization or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or Threonine anaerobic catabolism gene cluster (EC 2.3.1.8)

MetaCyc Pathways

• L-threonine degradation I (6/6 steps found)
• mixed acid fermentation (13/16 steps found)
• ethanolamine utilization (5/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• heterolactic fermentation (14/18 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• sulfoacetaldehyde degradation I (2/2 steps found)
• acetylene degradation (anaerobic) (4/5 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• pyruvate fermentation to acetate and lactate II (3/4 steps found)
• superpathway of L-threonine metabolism (13/18 steps found)
• pyruvate fermentation to acetate I (2/3 steps found)
• pyruvate fermentation to acetate VII (2/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (9/13 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (6/9 steps found)
• (S)-propane-1,2-diol degradation (3/5 steps found)
• sulfolactate degradation II (2/4 steps found)
• acetyl-CoA fermentation to butanoate (4/7 steps found)
• superpathway of sulfolactate degradation (3/6 steps found)
• superpathway of taurine degradation (3/6 steps found)
• methanogenesis from acetate (2/6 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (3/8 steps found)
• L-lysine fermentation to acetate and butanoate (4/10 steps found)
• purine nucleobases degradation II (anaerobic) (14/24 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (2/9 steps found)
• gallate degradation III (anaerobic) (3/11 steps found)
• superpathway of methanogenesis (5/21 steps found)
• superpathway of L-lysine degradation (15/43 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.8

Use Curated BLAST to search for 2.3.1.8

Sequence Analysis Tools

See G8AFR7 at UniProt or InterPro

Protein Sequence (483 amino acids)

>AZOBR_RS12500 bifunctional enoyl-CoA hydratase/phosphate acetyltransferase (Azospirillum brasilense Sp245)
MDRVIAPATPDFGKNGSAMIENVTFEEIQIGQQASLSRRLTMSDIELFATVSGDINPAHL
DEEYAADSQFHKVIGHGMWSGSLISAVLGTLLPGPGTIYMGQDLRFKRPVGLGDVITVTV
TAKEKHAEKNIVVFDCVARNQDGKEVVSGLAEVIAPTRKVRRAAHELPQVQVIRHDGHDE
LLGKTETLPPVPTAVVHPCDESSLKGAVEAAEANLIDPVLIGPASKIKSVAEAHGLDISR
YRIVDVAHSHASAETGVRLARSGECEAVMKGSLHTDELMAEVVRKETGLRTGRRLSHVFV
MNVPTYPRTLLITDAAINIYPTLEDKVDIVQNAIDLAKVLGVETPRVAILSAVETVNPKI
ASTLEAAALCKMADRGQIKGGILDGPLAFDNAISLEAARTKGIVSEVAGQADVLLVPDLE
AGNMLAKQLSFLANSDAAGIVLGARVPIILTSRADNVRTRLASCAVAVLAAAARRRGAAT
AAE