Protein Info for AZOBR_RS12300 in Azospirillum brasilense Sp245

Annotation: UDP-N-acetylmuramoylalanyl-D-glutamyl-2 6-diaminopimelate--D-alanyl-D-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 29 to 72 (44 residues), 42.4 bits, 1.1e-14
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 33 to 464 (432 residues), 392.2 bits, E=1.5e-121
• PF08245: Mur_ligase_M
  • amino acids 113 to 302 (190 residues), 171.8 bits, E=2.7e-54
• PF02875: Mur_ligase_C
  • amino acids 336 to 396 (61 residues), 27.3 bits, E=5.5e-10

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 73% identity to azl:AZL_020780)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (10/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (10/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (11/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (11/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (10/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

See G8AFM0 at UniProt or InterPro

Protein Sequence (498 amino acids)

>AZOBR_RS12300 UDP-N-acetylmuramoylalanyl-D-glutamyl-2  6-diaminopimelate--D-alanyl-D-alanine ligase (Azospirillum brasilense Sp245)
MSEAKTVLWTAEDAAAATSGQATRSWAATGVSIDSRKVAPGDLFVAIKGPNFDGHDFVAK
ALEAGAAAALVSTVPPGVPGDASLLAVEQDTLAALGDLGHVARLRTQAKVVGVTGSVGKT
STKEALRHVLSAQGRTFATEGSLNNHWGVPLSLARMPEDSEFGVFELGMNHAGELGPLSR
QVMPDVAVITTVEAAHLEFFDSVEAIADAKAEIFEGMNPNGVAVLNRDNPHFARLLAAAR
TQGLSRIWSFGSHADADARLVDCSLHATCSAVTAIVRGERIQYCLALPGRHWVMNSLAVL
LAVKALGADVAAAARALSTIAPVKGRGTRKRVQAARGAFTLIDESYNASPAAMTATFAVL
GKIDPGAGGRRLAVLGDMRELGDRADALHAALAEPLRAARVDAVYACGPHMRALFDRLPA
AMRGAWTETSAELAPLLAGAVRGGDVVLIKGSLGSRMATVVDALSALDTKDSKQTEKNNN
GSSPAADTDQTAAHGHKG