Protein Info for AZOBR_RS12160 in Azospirillum brasilense Sp245

Annotation: glycyl-tRNA synthetase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 3 to 693 (691 residues), 656.2 bits, see alignment E=4.4e-201 PF02092: tRNA_synt_2f" amino acids 4 to 551 (548 residues), 677.5 bits, see alignment E=1.7e-207 PF05746: DALR_1" amino acids 581 to 686 (106 residues), 38.2 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 64% identical to SYGB_MAGSA: Glycine--tRNA ligase beta subunit (glyS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 64% identity to mag:amb2650)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AFI9 at UniProt or InterPro

Protein Sequence (695 amino acids)

>AZOBR_RS12160 glycyl-tRNA synthetase subunit beta (Azospirillum brasilense Sp245)
MPELLIEFFSEEIPARMQARAADDLKRLVTEKLTANGLTFASAEAHSTPRRLALVIDGLP
ERTADVREEKKGPRVGSPEQAVAGFLKSAGLTSLDQCEQRDTGKGVFYFAVAEKKGRATA
EVLAEIIPAVMGDFPWPKSMRWGTGTVRWVRPLHSIIALFGGAVLEGSFDLGGAQGKLAF
GNSTRGHRFLSPDAFTVESFADYKAKLRAAHVVLDRAERKAKIKADAEALAAQEGLTLSP
DDGLLEEVAGLVEWPVALVGTIDEKFMDVPSEVLITSMRTHQKYFALLDKTGTMAPRFVV
VANTVTADGGKAIVAGNERVLRARLSDAKFFWDQDRKTKLEDRVSKLGAITFHAKLGTVA
EKVTRVQVLAAEIARAIGADADAATRAALLSKADLVTEVVGEFPEVQGIMGRYYALGEGE
NPAVAEAIAEHYKPLGPSDSCPTAPVSVAVALADKLDTLVGFFAIDEKPTGSKDPYALRR
AALGIIRLILENGLRLNLTQLFKAAHGAYSVGGFAAADGVATDLMAFFADRLKVTLRDQG
VRHDLVDAVFALGGEDDLVRLLARVKALQTFVGSEEGANLLAAYKRASNIVRIEEKKDGK
SYDQPVDPALLTLAEEKDLYGALSAAGETARPLLAKEDFTGTMAALARLRGPVDAFFDKV
TVNAEQADLRANRLRLLSQIRATLNAVADFSRIEG