Protein Info for AZOBR_RS10920 in Azospirillum brasilense Sp245

Annotation: carbamoyl phosphate synthase small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01368: carbamoyl-phosphate synthase, small subunit
  • amino acids 17 to 386 (370 residues), 463.4 bits, E=2.4e-143
• PF00988: CPSase_sm_chain
  • amino acids 18 to 143 (126 residues), 171.9 bits, E=7.8e-55
• PF00117: GATase
  • amino acids 206 to 383 (178 residues), 181.5 bits, E=2.1e-57

Best Hits

Swiss-Prot: 66% identical to CARA_BRADU: Carbamoyl-phosphate synthase small chain (carA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01956, carbamoyl-phosphate synthase small subunit [EC: 6.3.5.5] (inferred from 90% identity to azl:AZL_010540)

MetaCyc: 55% identical to carbamoyl-phosphate synthetase small subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of arginine and polyamine biosynthesis (17/17 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-citrulline biosynthesis (8/8 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (4/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See G8ANA2 at UniProt or InterPro

Protein Sequence (391 amino acids)

>AZOBR_RS10920 carbamoyl phosphate synthase small subunit (Azospirillum brasilense Sp245)
MAQSTLPAGDAGAYTGVLVLADGTVFKGRGIGAVGDSVGEVCFNTSMTGYQEILTDPSYA
GQIITFTFPHIGNTGANPEDIETVTPAARGLILRADVTDPSNWRATRHLDDWLKSYGLVG
LAGVDTRRLTRRIRDLGAPNGVVAHAPDGKFDLDALVAKAKGWPGLEGMDLAKDVSCRQT
YGWDEGAWTLGAGYATQESPRFHVVAIDFGAKRNILRCLAAAGCKVTVVPGTATVEDVMR
HKPDGVFLSNGPGDPAATGEYAVPTIKGLLDTGLPMFGICLGHQMLSLALGAKTKKMPLG
HRGANHPVKDLASGRVEITSQNHGFVVMEETLPADAEVTHVSLFDGTNEGIRLKNKPVFS
VQYHPEASPGPQDSHYLFDRFVALMDGKQPA