Protein Info for AZOBR_RS10910 in Azospirillum brasilense Sp245

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 TIGR01391: DNA primase" amino acids 4 to 418 (415 residues), 432.3 bits, see alignment E=9.1e-134 PF01807: zf-CHC2" amino acids 5 to 97 (93 residues), 110.4 bits, see alignment E=9.8e-36 PF08275: DNAG_N" amino acids 122 to 248 (127 residues), 127.6 bits, see alignment E=1e-40 PF13662: Toprim_4" amino acids 258 to 327 (70 residues), 56.8 bits, see alignment E=6.7e-19 PF01751: Toprim" amino acids 258 to 332 (75 residues), 39 bits, see alignment E=2.2e-13 PF13362: Toprim_3" amino acids 258 to 356 (99 residues), 29.5 bits, see alignment E=2.6e-10 PF13155: Toprim_2" amino acids 259 to 350 (92 residues), 54.7 bits, see alignment E=3.3e-18

Best Hits

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AN99 at UniProt or InterPro

Protein Sequence (644 amino acids)

>AZOBR_RS10910 DNA primase (Azospirillum brasilense Sp245)
MAFPPQFLEELRTRLALSDVVGKRLRLIRAGREYKAPCPFHNEKSPSFYVNDQKGFFHCF
GCGAHGDIIGFVMRHDNLAFPEAVEALAGEAGLPVPRATEEDKQRYERRKTLHDLVEQAA
RWFEQQLYAPAGRAGLEYFQRRGLDTDGMARFRLGFAPGDSGALRSQLLKQGFSEEDMVT
AGLLKRPEDGRTPYSFFRNRVIFPVTDRRGRVVAFGGRILEGDGPKYVNTADTPLFHKGT
LLYGLSRARQAAADGKPVIVAEGYMDVIALVRAGFEGAVAPLGTALTETQVQELWKLIPD
AEKVPFLCFDGDNAGRRAAWRAVERILPHLAPGHSARVAFLPEGEDPDSLIRLHGPRAMQ
EVLGEAIPLSEAVWRMETEGKPTDTPESKAAVKAALDARVGQIADRDVQSFYRTEMRRRV
DEAFAPPPRQRNERGPWVPGGSWQGGGQGGGRFGQQTRRHTPGLPGLRTAPPPGHRRRNP
GNLAAARERVLLAVIINHPELFDELGESLGMLPFPDRELEELRQAVIGLLAERRENDSGL
DAASLCRHLSSAGYDTIVRSLLSESTYVHAGFARPDASSLDAKRGWWPTWHHLHHRQVMA
DLREAEAALARDNSEANFARVVALQQEVIRSGMGMTDDEDLDDA