Protein Info for AZOBR_RS10625 in Azospirillum brasilense Sp245

Annotation: RNA polymerase sigma-70 factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 162 PF04542: Sigma70_r2" amino acids 5 to 70 (66 residues), 56.3 bits, see alignment E=4.4e-19 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 8 to 152 (145 residues), 78.6 bits, see alignment E=2.1e-26 PF07638: Sigma70_ECF" amino acids 93 to 151 (59 residues), 21.9 bits, see alignment E=3e-08 PF08281: Sigma70_r4_2" amino acids 97 to 149 (53 residues), 63.7 bits, see alignment E=1.9e-21 PF04545: Sigma70_r4" amino acids 102 to 151 (50 residues), 42.1 bits, see alignment E=9.8e-15

Best Hits

Swiss-Prot: 45% identical to ECFG_RHOPT: ECF RNA polymerase sigma factor EcfG (ecfG) from Rhodopseudomonas palustris (strain TIE-1)

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 87% identity to azl:AZL_012580)

Predicted SEED Role

"RNA polymerase sigma-54 factor RpoN" in subsystem Flagellar motility or Flagellum or Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (162 amino acids)

>AZOBR_RS10625 RNA polymerase sigma-70 factor (Azospirillum brasilense Sp245)
IIAQIEAEIPRLRRFARAMVRDATLADDLVQECLERALSRLHLWRPGSNLRAWLFTILRN
LHINGIRRRQAVVDIDGEGQAAIGAAHGGQFVRLELRDLKRALGLLPTEQREVVLLIGLE
GISYGEAADILGISIGTVKSRLSRGRRALRQLMEGHSPTDDD