Protein Info for AZOBR_RS10580 in Azospirillum brasilense Sp245
Annotation: protoheme IX farnesyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to COXX_RHIL3: Protoheme IX farnesyltransferase (ctaB) from Rhizobium leguminosarum bv. viciae (strain 3841)
KEGG orthology group: K02301, protoheme IX farnesyltransferase [EC: 2.5.1.-] (inferred from 80% identity to azl:AZL_012300)MetaCyc: 30% identical to heme O synthase (Escherichia coli K-12 substr. MG1655)
HEMEOSYN-RXN [EC: 2.5.1.141]
Predicted SEED Role
"Cytochrome c oxidase polypeptide I (EC 1.9.3.1)" in subsystem Terminal cytochrome C oxidases (EC 1.9.3.1)
MetaCyc Pathways
- aerobic respiration I (cytochrome c) (3/4 steps found)
- arsenite to oxygen electron transfer (1/2 steps found)
- aerobic respiration II (cytochrome c) (yeast) (2/4 steps found)
- heme a biosynthesis (2/4 steps found)
- arsenite to oxygen electron transfer (via azurin) (1/3 steps found)
- Fe(II) oxidation (2/6 steps found)
KEGG Metabolic Maps
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
- Methionine metabolism
- Oxidative phosphorylation
- Porphyrin and chlorophyll metabolism
- Riboflavin metabolism
- Terpenoid biosynthesis
- Tryptophan metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.9.3.1, 2.5.1.-
Use Curated BLAST to search for 1.9.3.1 or 2.5.1.- or 2.5.1.141
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8AN28 at UniProt or InterPro
Protein Sequence (309 amino acids)
>AZOBR_RS10580 protoheme IX farnesyltransferase (Azospirillum brasilense Sp245) MTDLSIERVSTGPVSGSTPGDYIELLKPRVMSLVVFTGLAGIVLAPGHIHPVLAAVAVLC IAVGAGASGAINMWYDRDIDAVMSRTIRRPIPSGRVEPENALAFGVILAAASVVVMGLAV NWAASALLAMTIGFYVFIYTMWLKRRTPQNIVIGGAAGAFPPMIGWAAVTGGVELPSILL FLLIFLWTPPHFWALALFRNGDYTRAGVPMMPVVAGPDATKRQMLAYTLVLLPVAVAPYF LGIGGPAYLIGSSLLGAFFVLCAVRVLRATDDKPAKQMFGFSILYLFLLFALLMGEHLVT GILTTGGGA