Protein Info for AZOBR_RS10210 in Azospirillum brasilense Sp245

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 transmembrane" amino acids 29 to 48 (20 residues), see Phobius details amino acids 60 to 83 (24 residues), see Phobius details amino acids 91 to 110 (20 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 150 to 171 (22 residues), see Phobius details amino acids 179 to 200 (22 residues), see Phobius details amino acids 229 to 252 (24 residues), see Phobius details amino acids 264 to 287 (24 residues), see Phobius details amino acids 295 to 312 (18 residues), see Phobius details amino acids 318 to 341 (24 residues), see Phobius details amino acids 353 to 376 (24 residues), see Phobius details amino acids 382 to 402 (21 residues), see Phobius details PF07690: MFS_1" amino acids 30 to 247 (218 residues), 110.5 bits, see alignment E=4.5e-36 amino acids 240 to 397 (158 residues), 38.7 bits, see alignment E=3e-14

Best Hits

Swiss-Prot: 46% identical to YNFM_ECOLI: Inner membrane transport protein YnfM (ynfM) from Escherichia coli (strain K12)

KEGG orthology group: K08224, MFS transporter, YNFM family, putative membrane transport protein (inferred from 71% identity to azl:AZL_014460)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AMT8 at UniProt or InterPro

Protein Sequence (404 amino acids)

>AZOBR_RS10210 membrane protein (Azospirillum brasilense Sp245)
IEPAGQGAAEPGTTYLVAGTPAYKRASRILFVAGFSTFAALYCVQPLMPEFTREFGVTPA
QSSLALSVSTGVLAFALLVAGAVSDRFGRKAIMALSLLASGTLGISGAMAPDWASLLLVR
ALEGMALSGLPAVAMAYVSEEVDPRSAGVTMGLYIGGTALGGMSGRALTALVADVASWRM
AMGVIGTLGIAAALAVWFALPASRNFQRRPAGMRELAEAWRGHLMDRGLLALFAMGFLSM
GAFVTVFNYIGFRLLAPPFDLRPAAVGAVFTVYLCGVVSSPLFGGLAVRLGRGRVLAAAA
AMMAAGLALMTPDSLWTMVPGIALFTFAFFGVHTIASGWIGQRAPVNRGQASAIYLFCYY
MGSTIAGTLGGVFWYAFGWAGVGGFVGLLLAVGLIVSLGLAVRR