Protein Info for AZOBR_RS10155 in Azospirillum brasilense Sp245

Annotation: NodQ bifunctional enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 14 to 406 (393 residues), 355.2 bits, E=7.7e-110
• PF00009: GTP_EFTU
  • amino acids 15 to 215 (201 residues), 160.1 bits, E=1.2e-50
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 434 to 612 (179 residues), 230.4 bits, E=1.9e-72
• PF01583: APS_kinase
  • amino acids 448 to 598 (151 residues), 213.8 bits, E=3.1e-67
• PF13671: AAA_33
  • amino acids 450 to 562 (113 residues), 28.8 bits, E=3.5e-10

Best Hits

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 81% identity to azl:AZL_009320)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (8/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (5/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.25, 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Sequence Analysis Tools

See G8AMS6 at UniProt or InterPro

Protein Sequence (627 amino acids)

>AZOBR_RS10155 NodQ bifunctional enzyme (Azospirillum brasilense Sp245)
IGTMDIRNELNGQLRIVIVGHVDHGKSTLIGRLLNDTGSLPDGKVEQVHEMSRKRGMPFE
WAFVMDALQAERDQGITIDTTQIHFKTEQRPYVIIDAPGHTEFLKNMVTGASQADAALLV
IDAAEGALEQSRRHAFLLHLLGVRQVAVAVNKMDRVDFDQYRFEAVSQEIRAYLAELGLH
TSTVIPIAARHGDNIVTRSDKAPWYDGPTVVEALDAFRPQQAPTELPLRFPLQDVYKPDD
RRILAGRIESGRLRVGDTLRFSPTGATATVVSIEGWNHSQPIVAAQAGQSIGITLDKRIF
AERGHVAHHEEGAPYVTHRLGVRAFWLTSEPLAVGKTYTLKITTGEHRVTVESITAVIDI
EDLSSTPAKAVHRNEVAEVVLRSRSPIAVDLASTLSRTGRGVLIDGHDVVGGCIVVEVDE
GPAASANTTEVSHAVTADERNAANGHKGGVLWLTGLSGAGKSTLAMALERALFDRGWQVF
VLDGDNVRNGLNAGLGFSAEDRAENIRRVAETAKLFADAGVLVIASLISPLQADRARARA
IGGERFHEVYVAADLATCESRDPKGLYRRARAGEIPEFTGVSAPYEAPESAELTIDTGTV
TPVEALGELLDYVDGAFGRNALKRGRV