Protein Info for AZOBR_RS08755 in Azospirillum brasilense Sp245

Annotation: valyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 885 transmembrane" amino acids 483 to 504 (22 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 5 to 863 (859 residues), 1152.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 17 to 570 (554 residues), 505.7 bits, see alignment E=2.3e-155 PF09334: tRNA-synt_1g" amino acids 42 to 191 (150 residues), 30.2 bits, see alignment E=5.1e-11 amino acids 358 to 575 (218 residues), 38.4 bits, see alignment E=1.7e-13 PF08264: Anticodon_1" amino acids 613 to 760 (148 residues), 136.6 bits, see alignment E=1.6e-43 PF10458: Val_tRNA-synt_C" amino acids 819 to 883 (65 residues), 71.6 bits, see alignment 1.4e-23

Best Hits

Swiss-Prot: 62% identical to SYV_CAUVC: Valine--tRNA ligase (valS) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 90% identity to azl:AZL_013080)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ALV7 at UniProt or InterPro

Protein Sequence (885 amino acids)

>AZOBR_RS08755 valyl-tRNA synthetase (Azospirillum brasilense Sp245)
LVMLEKTYRPAEVEEKHYRLWEETGAFAAKTESNGKPYTIMMPPPNVTGSLHMGHALTFT
LQDVLTRYNRMRGRDALWQPGTDHAGIATQMVVERNLAKEGKTRHDFGRDAFIDKVWEWK
AESGGTITRQLRRLGASPDWPRERFTMDEGLSRAVRKVFVELHKQGLIYKDKRLVNWDPK
LHTAISDLEVEQKEIKGNLWHFRYPIEGEADRFIVVATTRPETMLGDTGVAVHPEDERYK
DLIGKNVVLPLVGRLIPIVGDEYADPETGSGAVKITPAHDFNDFEVGRRNNLAAINIMDR
DARITGDEVPEAYRGLDRYEARKKVVAELEALGLLEKIEPHTHMVPHGDRSGVAIEPWLT
DQWYVDAATLAKPAIEAVETGKTVFVPKQWENTYFEWMRNIQPWCISRQIWWGHQIPAWY
GPDGHFFVEETEEAAIAAAKAHYGEDVALTRDQDVLDTWFSSALWPFSTLGWPDETPELA
RYYPTDVLVTGFDIIFFWVARMMMMGLHFMKDVPFRTVYIHALVRDEKGQKMSKSKGNVI
DPLELIDQYGTDALRFTLTAMAAQGRDIKLAVSRVEGYRNFATKLWNAARYTQMNGVAPV
PGFKPVGLTQTVNRWIVSALAEAAKKVGESIEAYKFNEAANTAYAFTWGTFCDWYLEFTK
PILSGTDEAAIAETRATTAWVLDEILHMLHPLMPFITEELWEQLSEDRANRLISARWPEH
GAELIDPAARDEMDWVVRAISSVRSMRSEMNVPPAAQIELKLKDAGPESLKRLDTHRDLI
LRMARLASVEPLSGPVPKSAVQAVLDEATLILPLEGIVDLDKERARLTKEIEKLSGEIKK
IDAKLSNEQFVAKAPEEVIEEQRDRRDTAEQARDKLQKALEMLAA