Protein Info for AZOBR_RS08320 in Azospirillum brasilense Sp245

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 TIGR00416: DNA repair protein RadA" amino acids 1 to 448 (448 residues), 558.9 bits, see alignment E=4.2e-172 PF18073: Rubredoxin_2" amino acids 8 to 35 (28 residues), 45.7 bits, see alignment (E = 1e-15) PF06745: ATPase" amino acids 71 to 141 (71 residues), 35.5 bits, see alignment E=1.7e-12 PF13481: AAA_25" amino acids 71 to 218 (148 residues), 55.8 bits, see alignment E=1.2e-18 PF13541: ChlI" amino acids 336 to 424 (89 residues), 30.5 bits, see alignment E=7.6e-11 PF05362: Lon_C" amino acids 361 to 426 (66 residues), 29.3 bits, see alignment E=1.6e-10

Best Hits

Swiss-Prot: 64% identical to RADA_BRUA2: DNA repair protein RadA (radA) from Brucella abortus (strain 2308)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 91% identity to azl:AZL_017020)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ALK6 at UniProt or InterPro

Protein Sequence (466 amino acids)

>AZOBR_RS08320 DNA repair protein RadA (Azospirillum brasilense Sp245)
LAKPTTRYVCQACGASFPKWAGRCDACGEWNTLVEEAAPEAAPKGLGVARGRRLDFVGLA
GSSEAPPRRMTRIEEFDRVCGGGLVPGSAILIGGDPGIGKSTLLLQAMARLSQEHRCAYV
SGEEAVDQVRLRAVRLGCAAAPVQLASATSVRDIVASLDGTDGPEVVVIDSIQTMYVDNL
DSAPGTVAQVRASAQELIRVAKRRGCVLLLVGHVTKEGTIAGPRVLEHMVDTVLYFEGER
GHQFRILRAVKNRFGATDEIGVFEMTDGGLGEVANPSALFLAERRGDVSGAAVFAGMEGT
RPVLVEVQALVAPSPLGTPRRAVVGWDSARLAMVLAVLEARCGVQIGANDVYLNVAGGLR
ITEPAADLAVAAALVSSLTGEPVPADAVVFGEIGLSGEVRAVGQSDARLKEAAKLGFSTA
IFPARRNGKKPGRGDTGLKSVELQQLSELLPLFQAGSGPRPPRGRD