Protein Info for AZOBR_RS08065 in Azospirillum brasilense Sp245

Annotation: urease subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 101 TIGR00192: urease, beta subunit" amino acids 1 to 100 (100 residues), 151.6 bits, see alignment E=3.2e-49 PF00699: Urease_beta" amino acids 3 to 99 (97 residues), 156.1 bits, see alignment E=1e-50

Best Hits

Swiss-Prot: 75% identical to URE2_CUPNH: Urease subunit beta (ureB) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K01429, urease subunit beta [EC: 3.5.1.5] (inferred from 88% identity to azl:AZL_021870)

MetaCyc: 71% identical to urease beta subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease beta subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ALE6 at UniProt or InterPro

Protein Sequence (101 amino acids)

>AZOBR_RS08065 urease subunit beta (Azospirillum brasilense Sp245)
MKPGEIIPAAGEIELNAGRDTVELDVANAGDRPIQVGSHYHFAETNGALTFDRETARGFR
LDIPAGTAVRFEPGQTRRVRLVAYAGNRVVIGFNGKVNGSL