Protein Info for AZOBR_RS07435 in Azospirillum brasilense Sp245

Annotation: molybdenum cofactor biosynthesis protein MoaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF03453: MoeA_N" amino acids 3 to 165 (163 residues), 155 bits, see alignment E=2e-49 PF00994: MoCF_biosynth" amino acids 179 to 313 (135 residues), 76.7 bits, see alignment E=2.4e-25 PF03454: MoeA_C" amino acids 326 to 398 (73 residues), 63.9 bits, see alignment E=1.9e-21

Best Hits

KEGG orthology group: K03750, molybdopterin biosynthesis protein MoeA (inferred from 71% identity to azl:AZL_016050)

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AL02 at UniProt or InterPro

Protein Sequence (405 amino acids)

>AZOBR_RS07435 molybdenum cofactor biosynthesis protein MoaA (Azospirillum brasilense Sp245)
MLQVGEARARILAAFTALPAETVPLPDALGRVLAEPAVARLTQPPFAAAAMDGWAVRSAD
IVPASADAPVTLRRIGESAAGHAFAGSVGAGEAVRIFTGAPLPAGADAVVMQEDCEDAGD
RVRVGRAVPAGRFIRPAGLDFTAGEELLPKGRLLTARDVALAAAANLPWLRVHRRPRVAV
LATGDEIALPGDPLGPSQIVSSNALGLCALVASQGGLAHNLGVAKDDPEHLAAMAAGAAG
CDLLVTTGGASQGEHDHVRDVLGGLSLDFYRVAMKPGKPLIFGTASGVPLLGLPGNPVST
GVAALLFLVPVLRRLQGLPAENATLTARLGAPLKANDDRTDFLRATLSTGADGEPVATPF
PRQDSAMMSRLARADALIVREPQATAAAVGDRVTVIPLSGGVLSL