Protein Info for AZOBR_RS07235 in Azospirillum brasilense Sp245

Annotation: biotin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 TIGR00121: biotin--[acetyl-CoA-carboxylase] ligase" amino acids 9 to 245 (237 residues), 155.7 bits, see alignment E=7.2e-50 PF03099: BPL_LplA_LipB" amino acids 15 to 139 (125 residues), 72.9 bits, see alignment E=3.6e-24 PF16917: BPL_LplA_LipB_2" amino acids 59 to 195 (137 residues), 64.1 bits, see alignment E=1.9e-21 PF02237: BPL_C" amino acids 201 to 247 (47 residues), 43.3 bits, see alignment 4.3e-15

Best Hits

KEGG orthology group: K03524, BirA family transcriptional regulator, biotin operon repressor / biotin-[acetyl-CoA-carboxylase] ligase [EC: 6.3.4.15] (inferred from 80% identity to azl:AZL_021340)

Predicted SEED Role

"Biotin--protein ligase (EC 6.3.4.9, EC 6.3.4.10, EC 6.3.4.11, EC 6.3.4.15)"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AKV9 at UniProt or InterPro

Protein Sequence (253 amino acids)

>AZOBR_RS07235 biotin (Azospirillum brasilense Sp245)
LRLPPGFRVMAFDSVGSTNDEAKAFARSGAAEGTIVWAKRQESGRGRRGRGWTSPEGNLY
SSTILRPSRPPAEAAQISFVAALAIADTAAAVLPDPDGVRCKWPNDVLVHGRKLSGILLE
SEAAARGGVSWLVLGVGINLRHFPEGTDYAATSLAAEGAPALQPAALLEIYAEQLARWYG
VWAEHGFGPVRAAWLKRARGLGEPIVVRLPDRTLTGTFADLDSDGVLLLDRDDGAGRQRI
AAGDVFFPPAVET