Protein Info for AZOBR_RS07200 in Azospirillum brasilense Sp245

Annotation: NADH:ubiquinone oxidoreductase subunit H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00146: NADHdh
  • amino acids 18 to 325 (308 residues), 441.1 bits, E=1e-136

Best Hits

Swiss-Prot: 77% identical to NUOH_RHOCS: NADH-quinone oxidoreductase subunit H (nuoH) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K00337, NADH dehydrogenase I subunit H [EC: 1.6.5.3] (inferred from 86% identity to azl:AZL_021410)

MetaCyc: 73% identical to MbhM (Pyrococcus furiosus)
Ferredoxin hydrogenase. [EC: 1.12.7.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• hydrogen production III (1/1 steps found)
• hydrogen production VIII (1/1 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• hydrogen production VI (1/2 steps found)
• superpathway of hydrogen production (1/2 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (6/9 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• superpathway of photosynthetic hydrogen production (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism
• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.12.7.2 or 1.6.5.3

Sequence Analysis Tools

See G8AKV2 at UniProt or InterPro

Protein Sequence (336 amino acids)

>AZOBR_RS07200 NADH:ubiquinone oxidoreductase subunit H (Azospirillum brasilense Sp245)
MAEFWSGFLLPLIIIVLKILAIVVPLLVAVAFMTYAERKIMGAMQLRQGPNIVGPFGLLQ
PFADGLKLFAKEQILPVGANRFVFYLAPMLTFFLALVAWAVIPFDYGMVLADINVGILYL
FAISSLGVYGIVMAGWASNSRYAFLGALRSAAQMVSYEVSMGLVIISVLLCVGSLNLTKI
VEAQETIWFAIPLLPMFVIFFISALAETNRSPFDLPEGESELVAGFMVEYSSAPFALFFL
GEYANMILMSAMTSILFLGGWLPPLPFAPFTWIPGPIWFALKIAFCLFVYVWVRATMPRY
RYDQLMRLGWKVFLPFSLLWVVLTAGVLVTFGWLPK