Protein Info for AZOBR_RS06715 in Azospirillum brasilense Sp245

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1115 transmembrane" amino acids 285 to 301 (17 residues), see Phobius details PF14701: hDGE_amylase" amino acids 11 to 111 (101 residues), 41.8 bits, see alignment E=1.6e-14 PF00128: Alpha-amylase" amino acids 25 to 102 (78 residues), 22.5 bits, see alignment 1.6e-08 PF11896: GlgE_dom_N_S" amino acids 472 to 655 (184 residues), 182.6 bits, see alignment E=1.9e-57 PF21702: GLGE_C" amino acids 1023 to 1110 (88 residues), 80 bits, see alignment E=2.5e-26

Best Hits

KEGG orthology group: None (inferred from 78% identity to azl:AZL_c02820)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AKJ8 at UniProt or InterPro

Protein Sequence (1115 amino acids)

>AZOBR_RS06715 alpha-amylase (Azospirillum brasilense Sp245)
MATAGPRIYNLFPTLVGPMRDWAGHLPRIQGMGFDWLFLNPIHYPGFSGSLYAVKDYYRL
HDRIQGGAPEHPDELLRGFIAEAGRHGQSVMLDLVINHTAKDAILVGEHPDWYRRDANGD
LYSPRAVDPVDPSRVTIWGDLAMLDYERAEVRAGLTDYWTRYLRHYIGLGVKGFRCDAAY
QIPAEVWKTLIERSREVDPQVKFFAETLGCTVEQVRDLCGAGFDFLFNSAKWWDFKSDWL
LDQYDEFRWIAPSIAFPESHDTDRLAAEVGSQDTERLAAQLKMHYLFAASFSTGVMMPVG
FEYGFTRKLDVVNTTPDDWEQPKLDLTGFIGAVNAMKAESPALNVEGPQRRVTSPHNPVI
GLIRETSGWANGSGEGCSVLLINPDENQPHAIDPGPLLASTGGGFADFEDVTPEAAPLPF
EPGRDLRLRPLEMRVFRARPAQSRPIELNHLGERGAEHDSATRAWMDELASRRVTIENVY
PELDGGRFPVKRVVGDVMEVWADIYTDGTFVLGAAVTYRPVDEEEWREVPMTFVDNDRWM
GKLPLTRNTRYQYSILAWRDVWESWRADFKKKNDAGLDVGLELIEGRRFVEHAVGLNEGE
GRAALERVVERMNSLQGAELTAYALSDEPRQAMATYGERQYLSRYGCDLEVYVDRTAARY
SAWFEIFPRSASPDPSRPGTFDDVSTMLPFIRGMGFDVLYFPPIHPIGRSFRKGRNNTLN
PGPNDPGVPYAIGASEGGHADIDPMIGDFEGFRRLVKEARRHGIEIALDFAVQCSPDHPW
IKSHPQWFYWRPDGTIRYAENPPKKYQDIVNVSFYRESYPDLWYALRDVVLFWCDEGVRI
FRVDNPHTKPFPFWEWMIREVQDRFPDALFLAEAFTRPKLMRRLAKIGFTQSYSYFTWRN
TKAELTEYLTELTQGESKDYMQPNFFANTPDILPPILVHGGRPAHMMRAVLAGTLSGVYG
LYAPYFVCEADPYPGKEEYNHSEKYEIRHWDWNKPGNIVDYVTRLNRIRAENPALHKFTN
LKFYNAYDDNILLYGKMTESKDNVILIAVNLDPHNGHGGTIEVPLWELGLDDGAHVQVED
LFTGQRFTWIGKFQHVWLDPQQNPAAIWRIRPPGR