Protein Info for AZOBR_RS06295 in Azospirillum brasilense Sp245

Annotation: queuine tRNA-ribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00430: tRNA-guanine transglycosylase
  • amino acids 6 to 366 (361 residues), 477.8 bits, E=2.1e-147
• TIGR00449: tRNA-guanine family transglycosylase
  • amino acids 6 to 360 (355 residues), 467.9 bits, E=2e-144
• PF01702: TGT
  • amino acids 14 to 361 (348 residues), 522.7 bits, E=2.4e-161

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

• queuosine biosynthesis I (de novo) (2/4 steps found)
• queuosine biosynthesis III (queuosine salvage) (2/5 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

See G8AKA0 at UniProt or InterPro

Protein Sequence (400 amino acids)

>AZOBR_RS06295 queuine tRNA-ribosyltransferase (Azospirillum brasilense Sp245)
MTGIGFELLKTDGRARRGRVSTAHGVINTPAFMPVGTAATVKAMTSDAVKSTGAEILLGN
TYHLMLRPTAERVGQLGGLHRFMNWDKPILTDSGGFQVMSLSDLRTMTEEGVTFKSHLDG
SKHHLTPERSIQIQHLLDSNITMCLDECTPFPATEAQAASSMRLSMRWAKRSKDAFVERP
GYGLFGIVQGGVYADLRAESVAALSDIGFDGYAIGGLAVGEGQETMFTINIRNARHAHRD
RPRYLMGVGRPSDLIGAVRRGVDMFDCVMPTRSGRTGQAFVRRGTINIRNARHAHDERPL
DEECGCPACRNHSRAYLHHLFKAGEMLGPMLLTWHNLHYYQDLMAELRQAIEDGRFDEVA
GALEARLNEGDIPATEDPLAHLKVKPAKPAKSAKKESADV