Protein Info for AZOBR_RS06255 in Azospirillum brasilense Sp245
Annotation: acyl-CoA synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 46% identical to AAE4_ARATH: Probable acyl-activating enzyme 4 (AEE4) from Arabidopsis thaliana
KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 69% identity to pmy:Pmen_3817)MetaCyc: 54% identical to short chain acyl-CoA synthetase (Pseudomonas putida KT2440)
Butyrate--CoA ligase. [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]
Predicted SEED Role
"3-methylmercaptopropionyl-CoA ligase (DmdB)"
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
- superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
- palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
- fatty acid salvage (5/6 steps found)
- valproate β-oxidation (6/9 steps found)
- 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
- 4-oxopentanoate degradation (5/9 steps found)
- octane oxidation (2/5 steps found)
- 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
- cannabinoid biosynthesis (6/13 steps found)
- sporopollenin precursors biosynthesis (4/18 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis II
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Butanoate metabolism
- Caprolactam degradation
- Ethylbenzene degradation
- Geraniol degradation
- Limonene and pinene degradation
- Propanoate metabolism
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.-
Use Curated BLAST to search for 6.2.1.- or 6.2.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8AK91 at UniProt or InterPro
Protein Sequence (551 amino acids)
>AZOBR_RS06255 acyl-CoA synthetase (Azospirillum brasilense Sp245) VTDARHNPYETDLDQNAANTVPLSPLSFLRRTAAVYPQRIAVIHGPVRRTWAETYERCVR LASALAKRGIGLGDTVAVMAPNTPESFEAHFGVPMTGAVLNALNIRLDAEALAFILEHGE AKVLLTDREFSGVISKAVHMLEPKRRPIVIDIDDPQAKGGELIGEQTYEQFLETGDPAYE WPMPADEWQAIALNYTSGTTGNPKGVVYHHRGAYLNAMGNVLTWAMPHHPVYLWTLPMFH CNGWCFPWTVTAMAGTNVCVRTITAKGIYDALADLGVTHMCGAPIIMGLIVNAPEDQKRE IPRGVKMMTAGAAPPAAVIEKIERMGFDVTHVYGLTEVYGPVTICAWHEHWNDLPLEERA ALKARQGVNYATLEGLMVADPNTLQPTRKDGVTMGEIFMRGNTVMKGYLKNPRATQEAFS GGWFHTGDLGVWHADGYIELKDRSKDIIISGGENISTIEVESVLYKHPDIVEAAVVARPD EKWGETPCAFVTVKEGKQLTEAEVIAYCREHLAHFKCPRTVVFTALPKTSTGKIQKYVLR DQARALNGAKV