Protein Info for AZOBR_RS05435 in Azospirillum brasilense Sp245

Annotation: recombinase RecD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 730 TIGR01448: helicase, RecD/TraA family" amino acids 17 to 724 (708 residues), 702.5 bits, see alignment E=2.6e-215 PF14520: HHH_5" amino acids 103 to 152 (50 residues), 45.6 bits, see alignment 3.9e-15 PF14490: HHH_4" amino acids 154 to 242 (89 residues), 106.1 bits, see alignment E=3.4e-34 PF13604: AAA_30" amino acids 334 to 513 (180 residues), 149.7 bits, see alignment E=4.2e-47 PF13245: AAA_19" amino acids 338 to 469 (132 residues), 158.4 bits, see alignment E=5.7e-50 PF01443: Viral_helicase1" amino acids 351 to 466 (116 residues), 21.1 bits, see alignment E=1.2e-07 PF03266: NTPase_1" amino acids 351 to 379 (29 residues), 24.2 bits, see alignment (E = 1.4e-08) PF05127: Helicase_RecD" amino acids 352 to 448 (97 residues), 24.2 bits, see alignment E=1.4e-08 PF18335: SH3_13" amino acids 574 to 636 (63 residues), 70.8 bits, see alignment E=3.4e-23 PF13538: UvrD_C_2" amino acids 653 to 700 (48 residues), 65.8 bits, see alignment 1.3e-21

Best Hits

KEGG orthology group: K03581, exodeoxyribonuclease V alpha subunit [EC: 3.1.11.5] (inferred from 73% identity to gdi:GDI_0965)

Predicted SEED Role

"RecD-like DNA helicase YrrC"

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AJP9 at UniProt or InterPro

Protein Sequence (730 amino acids)

>AZOBR_RS05435 recombinase RecD (Azospirillum brasilense Sp245)
ISGHAPPAAGTPAREPLSGLVERVTFHSPETGFCVLRLKVRGQRDLVTLVGHAATIGAGE
FVQASGVWVNDRNHGLQFKADFLRATPPTTVEGIEKYLGSGLIRGIGPVYAKKMVKAFGE
AVFDVIEQEPDRLRQVTGIGPKRAQRIIAGWADQKVIREIMIFLHSHGVGTSRAVRIFKT
YGPDAIRLITENPYRLARDIRGIGFKTADAVAARLGIEKTAMIRARAGIGYALAEATDQG
HCGVPVAELIPLAVKLLEIDASILETAAELERQDGAVVADSLGGEPCLFLASLYRAEQAI
AERLRRLAGGPVPWPEIDAEKAIPWVEAKAGITLAEGQREALRQAATSKLLVITGGPGVG
KTTLVNSILTMLKAKGVSIALAAPTGRAAKRLSESTGLEARTIHRLLETDPQAGGFKRDE
TNPLDCDLLVVDETSMVDVPLMNALLRAVPGRAALLLVGDVDQLPSVGPGQVLGDVIGSG
AVPVVRLTEIFRQAATSRIIVNAHRINAGQMPEIPKTGEDSDFYFVEAATPEAGVAKLIE
MVRDRIPRRFGADPVRDVQILCPMNRGGLGARSLNIELQRVLNPPGESRVERFGWTFGPG
DKVMQVENNYDKEVYNGDLGIVSAVDAEEGVLTATFDGRPVTYDFGELDEVVLAYATTIH
KSQGSEYPVVVIPVVTQHYTMLHRNLIYTGVTRGKRLVVMLGQRRALAMAVRGTPGRRRW
TKLGEWLAPL