Protein Info for AZOBR_RS05265 in Azospirillum brasilense Sp245

Annotation: formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 352 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details transmembrane" amino acids 99 to 121 (23 residues), see Phobius details amino acids 142 to 163 (22 residues), see Phobius details amino acids 186 to 206 (21 residues), see Phobius details amino acids 243 to 264 (22 residues), see Phobius details amino acids 276 to 298 (23 residues), see Phobius details TIGR01583: formate dehydrogenase, gamma subunit" amino acids 132 to 332 (201 residues), 160.4 bits, see alignment E=2.8e-51 PF01292: Ni_hydr_CYTB" amino acids 134 to 313 (180 residues), 80.5 bits, see alignment E=7e-27

Best Hits

KEGG orthology group: K00127, formate dehydrogenase, gamma subunit [EC: 1.2.1.2] (inferred from 72% identity to azl:AZL_007330)

Predicted SEED Role

"Formate dehydrogenase -O, gamma subunit (EC 1.2.1.2)" in subsystem Anaerobic respiratory reductases or Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AJK9 at UniProt or InterPro

Protein Sequence (352 amino acids)

>AZOBR_RS05265 formate dehydrogenase (Azospirillum brasilense Sp245)
MTSNHSKGGVGRTLHIVLLGVAMLALTLVMANVSAASAAEAFPPVASQPADGTSKTDMWR
GIRSGEQGYVSIPNKSAGVLVQSEGELWRSVRNGPLSTYGGWALGGMIGLLVLFFLVRGR
IRIDGQKTGRTIQRFNAFERGVHWLTAGSFVILAFTGLNVLYGRYTVLPLLGPEAFATMT
QYGKFAHNYLGFAFMLGIVLIFVMWVKHNIPERNDITWALKAGGLFSKGVHPAAKKFNAG
QKVIFWATVLGGAALGFTGVQLLWPFSFASMADMQLYQLIHAAVAVVLTAVIIAHIYIGS
VGMEGAFDAMGTGDVELQWAREHHSLWVQEVTGETPGRHGGHHHGRHRAPAE