Protein Info for AZOBR_RS04810 in Azospirillum brasilense Sp245

Annotation: shikimate 5-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01488: Shikimate_DH
  • amino acids 2 to 54 (53 residues), 25 bits, E=5.3e-09
• PF00106: adh_short
  • amino acids 7 to 196 (190 residues), 199.4 bits, E=1.3e-62
• PF02719: Polysacc_synt_2
  • amino acids 9 to 77 (69 residues), 20.3 bits, E=8.1e-08
• PF08659: KR
  • amino acids 9 to 168 (160 residues), 55.8 bits, E=1.7e-18
• PF13561: adh_short_C2
  • amino acids 13 to 258 (246 residues), 201.5 bits, E=4.7e-63

Best Hits

Swiss-Prot: 41% identical to GDH_AGRFC: Galactitol 2-dehydrogenase (Atu3164) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03366, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 85% identity to azl:AZL_009050)

MetaCyc: 41% identical to galactitol 2-dehydrogenase (Agrobacterium fabrum C58)
Galactitol 2-dehydrogenase. [EC: 1.1.1.16]

Predicted SEED Role

"2,3-butanediol dehydrogenase, S-alcohol forming, (S)-acetoin-specific (EC 1.1.1.76)" in subsystem Acetoin, butanediol metabolism (EC 1.1.1.76)

MetaCyc Pathways

• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• superpathway of (R,R)-butanediol biosynthesis (4/5 steps found)
• (R,R)-butanediol biosynthesis (1/1 steps found)
• (S,S)-butanediol biosynthesis (1/1 steps found)
• (R,R)-butanediol degradation (1/1 steps found)
• (S,S)-butanediol degradation (1/1 steps found)
• superpathway of 2,3-butanediol biosynthesis (3/6 steps found)
• D-altritol and galactitol degradation (1/4 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Galactose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.16 or 1.1.1.303 or 1.1.1.4 or 1.1.1.76

Sequence Analysis Tools

See G8AJA1 at UniProt or InterPro

Protein Sequence (261 amino acids)

>AZOBR_RS04810 shikimate 5-dehydrogenase (Azospirillum brasilense Sp245)
MSNLKDKSIIVTGAGRGIGATMARALAADGARLIIADRTEEDAKTVAESIRAKGGDAVAV
TVDVRDRAAVRRMIDTAVTSFGRLDVLFNNAGVAQTKPFLDITEEDWRFVTDVNALGVLI
GMQEAIKTFRAQGGGGKIINTASIAGKQGYEPLAHYSASKFAVVALTQAAARAFGKEKIT
ANAICPGVVATEMWKIIDKGFRDTGLTTAEDEAFNHFAAGAVLGRPSTAEDLVGVARFLA
SSDSDFMTGQSLLVDGGMVFA