Protein Info for AZOBR_RS04335 in Azospirillum brasilense Sp245

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 PF18072: FGAR-AT_linker" amino acids 14 to 54 (41 residues), 54.8 bits, see alignment 1.6e-18 TIGR01736: phosphoribosylformylglycinamidine synthase II" amino acids 19 to 729 (711 residues), 933.6 bits, see alignment E=3.8e-285 PF00586: AIRS" amino acids 75 to 190 (116 residues), 96.4 bits, see alignment E=2.2e-31 amino acids 441 to 558 (118 residues), 83.6 bits, see alignment E=2e-27 PF02769: AIRS_C" amino acids 203 to 356 (154 residues), 132.9 bits, see alignment E=1.7e-42 amino acids 573 to 706 (134 residues), 67.3 bits, see alignment E=2.7e-22

Best Hits

Swiss-Prot: 69% identical to PURL_RHORT: Phosphoribosylformylglycinamidine synthase subunit PurL (purL) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 84% identity to azl:AZL_022860)

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.3

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AIZ7 at UniProt or InterPro

Protein Sequence (738 amino acids)

>AZOBR_RS04335 phosphoribosylformylglycinamidine synthase (Azospirillum brasilense Sp245)
VTTAQQPAVSLEMAREFGLSEGEYNNALEILGRQPTYTELGIISVMWSEHCSYKSSKKWL
MTLPTTGPQVICGPGENAGVVDAGDGDAIIFKMESHNHPSYIEPFQGAATGVGGILRDVF
TMGARPIANMNALRFGAPEHPKTRHLVSGVVAGIAHYGNCVGVPTIGGETNFHPAYNGNI
LVNAMTVGVAKADRIFYSAAAGIGNPVVYVGSKTGRDGIHGATMASAEFSEDSEEKRPTV
QVGDPFTEKLLIEACLELMETDAIVAIQDMGAAGLTSSSVEMAGKGGLGIELVLDDVPMR
EEGMTPYEIMLSESQERMLIILKPGREEVAKAIFDKWELDFAVIGTLTDNGKLVIKMHGQ
TWCDMPIAPVSNASPLYDRPWEPTPAAAELEDAVRALPADKSLGDILETIMSCPDQASKR
WIWEQYDSLVNGDTRFMSGQADAGVVRLPGQSKGIAVTTDCTPRYCLADPVRGGAQAVAE
AYRNLSAVGALPLAVTDNMNFGNPEKPRIMGQFVGAIQGMADACRALDFPVVSGNVSLYN
ETNGEAILPTPAIGAVGLLPDAMIAVGIAFKNEGDSILLVGEGTGHLGQSLYLREILGRE
DGPPPPVDLAVERRNGEFVRGLIAEGLVVSSHDIQDGGLLVTVAEMAMAGGIGANLSGLD
GADAGTLFGEDQGRYVLAVRADKAAAVQEKAKAAGVPVSVLGKTNGTALTGRGGEAITIA
RLKKANEAWLPGYMAAAE