Protein Info for AZOBR_RS04265 in Azospirillum brasilense Sp245

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 PF00682: HMGL-like" amino acids 6 to 265 (260 residues), 150.1 bits, see alignment E=9e-48 TIGR00977: citramalate synthase" amino acids 6 to 527 (522 residues), 666.3 bits, see alignment E=1.3e-204 PF08502: LeuA_dimer" amino acids 380 to 521 (142 residues), 93.1 bits, see alignment E=1.4e-30

Best Hits

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 85% identity to azl:AZL_024210)

Predicted SEED Role

"(R)-citramalate synthase (EC 2.3.1.182)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 2.3.1.182)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.1.182 or 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AIY1 at UniProt or InterPro

Protein Sequence (534 amino acids)

>AZOBR_RS04265 2-isopropylmalate synthase (Azospirillum brasilense Sp245)
MTGERIYLYDTTLRDGAQTQDVDFSVADKIAIARDLDRLGIDYVEGGWPGANPTDDQFFA
EAPDLARSTFTAFGMTRRPGRSTANDPQLAALIQSKARAVCIVGKTWDFHVDVALAIPRE
ENIELIADSIAALKAAKGEALFDAEHFFDGYKRNPAYAASCIKAAYEAGARWVVLCDTNG
GTLPHEIDEIVRQVIDEHGIPGDRLGIHCHNDTENAVANSLAAVRAGVRMVQGTINGLGE
RCGNANLVSLIPSLMLKMGCDVGIKPADLPLLTEISRKFDERLNRAPNRHAPYVGASAFA
HKGGLHVSAVEKDPSSYEHVEPEAVGNRRQIVMSDQAGRSNVIARLREMGMAVDPKDERL
NRLLDIVKQRDTEGYAYDTAEASFELLVRRELGEVPDYFTLKRFHVTDERRYNAKGELVV
ESEAVVRVKIGGKEYHEVASGNGPVNALDTAMRKALEPLYPLLDGVKLSDYRVRILAAKD
GTGAMPRVLIRSRNGDGQEWSTLGVSTNIIEASMEALVDSYTFKLFKGGATPRS