Protein Info for AZOBR_RS03980 in Azospirillum brasilense Sp245

Annotation: primosome assembly protein PriA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 717 PF17764: PriA_3primeBD" amino acids 1 to 92 (92 residues), 68 bits, see alignment E=1.6e-22 PF04851: ResIII" amino acids 185 to 317 (133 residues), 52.1 bits, see alignment E=2.3e-17 PF00270: DEAD" amino acids 206 to 350 (145 residues), 70.8 bits, see alignment E=3.5e-23 TIGR00595: primosomal protein N'" amino acids 209 to 714 (506 residues), 650.4 bits, see alignment E=9.5e-200 PF18319: PriA_CRR" amino acids 435 to 461 (27 residues), 37.2 bits, see alignment (E = 6.8e-13) PF18074: PriA_C" amino acids 623 to 713 (91 residues), 68.3 bits, see alignment E=2.5e-22

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 86% identity to azl:AZL_024970)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AIR9 at UniProt or InterPro

Protein Sequence (717 amino acids)

>AZOBR_RS03980 primosome assembly protein PriA (Azospirillum brasilense Sp245)
VLLPLPLREAYDYRVPAGMTLVPGDYVEVPLGPRRVLGVVWGDGAGVVEAARLKPVVRRF
DVPPMPEVERKFVEWVAAYTMTPPGHVLRMAISVPSALEPAKAMLAYLRKPDAEPPPGFK
MTDPRRRVLALLDDGPPRTPAELAEEAGCGVTVVRGLAEAGLLEPVLLQPTKLGRPDCHR
PGPTLSADQEAAAADLRARVESGVYSTVLLDGVTGSGKTEVYYEAIAAALKAGKQALVLL
PEIALSAQWLERFARRFGAPPAEWHSELTGAQRRDTWRAVANGEVPVVVGARSALFLPYK
NLGVIIIDEEHDSAYKQEEGSIYHARDMAVARAHIGGIPTVLVSATPSLETKVNADSNRY
ARIELPGRHGGAVLPDVELVDLRKDRPPARHWLAPSLKKAIEQTLAEKEQVMLFLNRRGY
APLTLCRACGHRLQCPNCTAWLVEHRLARKLQCHHCGLSQPLSPTCPECGEEGTLAACGP
GVERIAEEVAELFPDARAAIMASDTLFGPRAIQEMVRRIADHELDILIGTQVMAKGHHFP
MLTLVGVVDADLGLAGGDLRAGERTYQLLHQVAGRAGRGERPGRVMLQTYMPEHPVMQAL
AAGDRDGFYQLEAEMRLEAGMPPFGRLAALIVSGEDAAAVEKLSIALGRAAPRSDTVAVL
GPAPAPLALLRGRHRRRLLLKAPRTVQVQPLIAQWLEQVEIPPNVRVQVDVDPYSFL