Protein Info for AZOBR_RS03960 in Azospirillum brasilense Sp245

Annotation: dihydrolipoamide dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 6 to 466 (461 residues), 586 bits, E=2.5e-180
• PF07992: Pyr_redox_2
  • amino acids 6 to 328 (323 residues), 260.5 bits, E=9e-81
• PF01134: GIDA
  • amino acids 7 to 67 (61 residues), 24.5 bits, E=5.4e-09
• PF12831: FAD_oxidored
  • amino acids 7 to 48 (42 residues), 38.2 bits, 4.6e-13
• PF00890: FAD_binding_2
  • amino acids 7 to 43 (37 residues), 31.8 bits, 3.7e-11
• PF13450: NAD_binding_8
  • amino acids 10 to 45 (36 residues), 29.7 bits, 2.6e-10
• PF00070: Pyr_redox
  • amino acids 175 to 249 (75 residues), 73.8 bits, E=5.4e-24
• PF02852: Pyr_redox_dim
  • amino acids 347 to 456 (110 residues), 145.1 bits, E=3.7e-46

Best Hits

Swiss-Prot: 61% identical to DLDH_PEA: Dihydrolipoyl dehydrogenase, mitochondrial (LPD) from Pisum sativum

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 84% identity to azl:AZL_025020)

MetaCyc: 61% identical to glycine cleavage system L protein 1 (Arabidopsis thaliana col)
GCVMULTI-RXN [EC: 1.4.1.27]

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)" in subsystem TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (2/3 steps found)
• glycine degradation (2/3 steps found)
• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• folate transformations II (plants) (7/11 steps found)
• photorespiration II (6/10 steps found)
• folate transformations I (8/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.4.1.27 or 1.8.1.4

Sequence Analysis Tools

See G8AIR1 at UniProt or InterPro

Protein Sequence (466 amino acids)

>AZOBR_RS03960 dihydrolipoamide dehydrogenase (Azospirillum brasilense Sp245)
MAESTFDVVVVGGGPGGYVCAIRAAQLGFKVACVEKRSALGGTCLNVGCIPSKALLAASE
KYEEAKHGLAKFGIKVDGVELDLPGMLSHKDKVVKENTGGIEFLFKKNKIAWLKGAGRIT
APNTVEVEGVGTITASKAIVIATGSEVTPLPGIEIDEQKIVSSTGALELPEVPKRLVVIG
GGVIGLELGSVWGRLGAEVTVVEFLDRILPTMDGEVSKQMQRILGKQGMTFKLGSKVTGA
KVTNTGVTLSVEPAAGGTAEEIKADVVLVAIGRRAFTNGLGLDAVGVEMDNRGRVKIGKH
FETNVPGIYAIGDVVEGPMLAHKAEEEGVALAELLAGQAGHVNHDLVPGVVYTWPEVAAV
GKTEEELKAAGTAYKAGKFPFTANGRARASGTTDGFVKILADARTDKVLGVHMVGPNVSE
MVAELAVAMEFSASAEDIARTCHAHPTLSEVTKEAALAVDGRALHI