Protein Info for AZOBR_RS03745 in Azospirillum brasilense Sp245

Annotation: DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1094 TIGR00594: DNA polymerase III, alpha subunit" amino acids 5 to 784 (780 residues), 504.3 bits, see alignment E=3.8e-155 PF02811: PHP" amino acids 7 to 120 (114 residues), 44.5 bits, see alignment E=5.4e-15 PF07733: DNA_pol3_alpha" amino acids 274 to 528 (255 residues), 296.6 bits, see alignment E=4.8e-92 PF17657: DNA_pol3_finger" amino acids 531 to 695 (165 residues), 195.2 bits, see alignment E=1.3e-61 PF14579: HHH_6" amino acids 770 to 902 (133 residues), 60.9 bits, see alignment E=2.8e-20 PF01336: tRNA_anti-codon" amino acids 985 to 1060 (76 residues), 29 bits, see alignment 2.1e-10

Best Hits

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AIL3 at UniProt or InterPro

Protein Sequence (1094 amino acids)

>AZOBR_RS03745 DNA polymerase (Azospirillum brasilense Sp245)
MTPYAELQVSTSFTFLEGASHPDELALTAASLGHAAVAVTDRNSLAGVVQAHAAARKHGL
RLIVGCRLDLTDADSLLAYPTDRAAYARLSRLLTLGKRRAPKGACFITRADVLDHAEGML
FLLVPPDNRDPAFVHALRGWRNDLGDRLHLAASHRYRGDDQRRLSWLAGLAEAERVPLVA
TNDVLYHTPARRPLADVMTCIRSGTTIDEAGWRLSANAERHLKAGVEMARLFHRHPDAVA
RTVEIAAACRFSLEELRYEYPDEVAEDGRSPQETLTNLTWAGAVQRYPDGVPETVAQTLH
RELALIGRLDYAPYFLTVHDIVRHARSQDILCQGRGSAANSAVCYCLGITSVDPATTRLL
FERFISSARNEPPDIDVDFEHEKREKVIQYIYEKYGRERAGLTATVIRYRARGAIREVGK
AMGLSADLVARLSSSVWGWSRHGVDGERARQYGFDPDDPRLRQTLELAQELIGFPRHLSQ
HVGGFVITRGPLSDLCPIANAAMKDRTTIEWDKDDIDALGILKVDVLALGMLTCLHRGFD
LLKQHYDSDLTLATVPQGDPATYDMLCRADSLGVFQVESRAQMSMLPRLRPKKFYDLVIQ
VAIVRPGPIQGDMVHPYLRRRSGEEVVTYPSKDLEAVLDRTLGVPLFQEQAMQIAIVGAG
FSAEDADRLRRAMATFRKTGEVQLFRDRFIEGMVGKDYDRAFAERCFQQIEGFGEYGFPE
SHAASFALLAYVSAWMKCHHPDVFAAALLNSQPMGFYAPAQIVRDAREHGVIVLPPDVNA
SDWDCTLEPLSKTETHTLSPLGRGEGEGDAHVPNVPEQRIPLTPTLSPEGRGSTGNIRHA
LRLGLRLVRGMDEEDAHQLVLCRGAGYRDPYDLWRRARMPVSGLERLAKADAFRSVGLDR
RAALWAVKALGAQPLPLFAGLADSAQDEPQALLPAMALGEHVVMDYGSLCLSLKAHPMAL
LRDGFAGVAPTERLGKVRAGTRLTVAGLALVRQRPGSAEGVVFITLEDETGIANLVIMPD
VFEQFRKAIIGARLIAATGRVERRGKPNLEGGEVIHLRVERLSDLTHRLRDLTDPDALPN
RSAAAVFPEGRNFR