Protein Info for AZOBR_RS02940 in Azospirillum brasilense Sp245

Updated annotation (from data): large component of pyruvate transporter (actP-like)
Rationale: Specifically important for pyruvate utilization.
Original annotation: actetate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 572 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 41 to 60 (20 residues), see Phobius details amino acids 81 to 103 (23 residues), see Phobius details amino acids 109 to 128 (20 residues), see Phobius details amino acids 155 to 175 (21 residues), see Phobius details amino acids 187 to 208 (22 residues), see Phobius details amino acids 218 to 238 (21 residues), see Phobius details amino acids 269 to 292 (24 residues), see Phobius details amino acids 304 to 331 (28 residues), see Phobius details amino acids 378 to 405 (28 residues), see Phobius details amino acids 427 to 446 (20 residues), see Phobius details amino acids 452 to 472 (21 residues), see Phobius details amino acids 483 to 503 (21 residues), see Phobius details amino acids 522 to 540 (19 residues), see Phobius details TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 69 to 491 (423 residues), 206.9 bits, see alignment E=2.8e-65 PF00474: SSF" amino acids 69 to 491 (423 residues), 395.6 bits, see alignment E=1.3e-122

Best Hits

Swiss-Prot: 67% identical to ACTP_YERPE: Cation/acetate symporter ActP (actP) from Yersinia pestis

KEGG orthology group: K14393, cation/acetate symporter (inferred from 84% identity to azl:AZL_001530)

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AHY6 at UniProt or InterPro

Protein Sequence (572 amino acids)

>AZOBR_RS02940 large component of pyruvate transporter (actP-like) (Azospirillum brasilense Sp245)
MRSLVNRVGVAAVAAGALIPASVHAAAVEGAVQKQATNFSAIVMFLVFVLATLGITYWAA
RRTKSAKDFYAAGGGITGFQNGLAIAGDYMSAASFLGIAGLVYTSGFDGLIFSVGWLVGW
PIILFLVAERLRNLGKYTFADVASYRFQQTPMRTMAACGSLATVTFYLIAQMVGAGKLIQ
LLFGMDYLWAVVIVGVLMIAYVTFGGMLATTWVQIIKAVLLLSGASFMAFAVLAKFGFSP
EAMFSTAVQVHPKATGIMAPGALITDPVSAISLGMALMFGTAGLPHILMRFFTVSDAKEA
RKSVFYATGFIGYFYILTFIIGFGAIVLLLAPDATGAYPFLDAAKLAAAGGKPNPSMIIG
GSNMAAIHTAHAVGGDLFFGFISAVAFATILAVVAGLTLAGASAVSHDLYASVIAKGRAS
EHDEIRVSKITTVIIGIVSIFLGIAFENQNVAFMVGLAFVIAASANFPVLLMSMFWSRMT
TRGAVLGGWIGLVSSVSLLIMGPTVWKSVLGNPAALFPYDNPGVFTIPLSFLAIWFFSIT
DNSKAAQDEREAYKAQYIRSQTGLGAEGASAH