Protein Info for AZOBR_RS01405 in Azospirillum brasilense Sp245

Annotation: translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 911 PF08364: IF2_assoc" amino acids 20 to 55 (36 residues), 36.9 bits, see alignment (E = 1.5e-12) TIGR00487: translation initiation factor IF-2" amino acids 325 to 909 (585 residues), 836.7 bits, see alignment E=1.2e-255 PF04760: IF2_N" amino acids 333 to 382 (50 residues), 65.7 bits, see alignment 1.1e-21 TIGR00231: small GTP-binding protein domain" amino acids 411 to 565 (155 residues), 115.3 bits, see alignment E=2.4e-37 PF00009: GTP_EFTU" amino acids 413 to 569 (157 residues), 120.7 bits, see alignment E=2.7e-38 PF01926: MMR_HSR1" amino acids 413 to 519 (107 residues), 43.2 bits, see alignment E=1.8e-14 PF00071: Ras" amino acids 414 to 567 (154 residues), 24.8 bits, see alignment E=6.8e-09 PF00025: Arf" amino acids 415 to 562 (148 residues), 23.9 bits, see alignment E=1.2e-08 PF11987: IF-2" amino acids 685 to 801 (117 residues), 147 bits, see alignment E=1e-46 PF03144: GTP_EFTU_D2" amino acids 832 to 899 (68 residues), 35.9 bits, see alignment 3.6e-12

Best Hits

Swiss-Prot: 64% identical to IF2_RHORT: Translation initiation factor IF-2 (infB) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 79% identity to azl:AZL_028590)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AFH5 at UniProt or InterPro

Protein Sequence (911 amino acids)

>AZOBR_RS01405 translation initiation factor IF-2 (Azospirillum brasilense Sp245)
MTDSNDQDQKKVLHLSGSGKGKLELKKPVETQVRQSFSHGRSKPVTVEVKRKRAVEKGAL
PGVADGGAAARQAAQGIPVRGVPGQRQRGGGGGAVRQLTNQEREARIRALQGAAEDNRRR
AEEEAEAAVLAAEAAARAAEEAASQPAQVVEEEPEILDAETLRQRELAELRGIEEAERAK
AQEAERRRQEEEAKRKEAEEAKRKDTEQTRPQGAGARPAAGGAGARTAATTTEAPAARGP
GAPAAPRFGEEEDDRRGKPGNKKAPAPAPVRKAAPGADRRKGSKMTVSQALSDEGGERTR
SLAAVRRARERERLRQMSRQETQKVTRDVVLPEVITVQELANRMAERGADVIKQLMRMGV
MATINQTIDADTAELIIAEFGHRVRRVSEADVEVGLRLNDDAETALVPRPPVVTIMGHVD
HGKTSLLDALRQTDVVSREAGGITQHIGAYQVQLESGAKITFIDTPGHAAFTEMRARGAN
VTDVVVLVVAANDGVMPQTIEAIHHAKAAKVPIIVAINKIDLPDAKPDRVRQELLQHELV
VEELGGDIQTVEVSAKAKRNLDKLEEAILLQAEILELKANPERTAEGVVVEAKLERGRGS
VATVLVQRGTLKVGDVFVTGSEWGRVRALVNDRGQSVEQAAPASPVEVLGLNGTPLAGDD
FTVVESEARAREIAEFRQRKKREAANAASARGSLQDMFSRIQAGEAKELPVVIKGDVQGS
IEAISNALEKLTAENTEVKVRVLHASVGAINESDITLANASNAMVIGFNVRANPQARDMA
KRDSVEIRYYSIIYNVIDDVKAALTGMLSPTLRERFIGYAEIREVFNITKVGKVAGCMVT
QGTVKRGAGCRLLRDNVVIHEGTLKTLKRFKDEVKEVREGYECGMAFENYDNILAGDIIE
AFEIEEVAREL