Protein Info for AZOBR_RS01310 in Azospirillum brasilense Sp245

Annotation: lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00056: Ldh_1_N
  • amino acids 1 to 139 (139 residues), 128.4 bits, E=2.1e-41
• TIGR01771: L-lactate dehydrogenase
  • amino acids 5 to 303 (299 residues), 341.5 bits, E=2.3e-106
• PF02866: Ldh_1_C
  • amino acids 142 to 310 (169 residues), 106.9 bits, E=1.2e-34

Best Hits

Swiss-Prot: 66% identical to LDH_THET2: L-lactate dehydrogenase (ldh) from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)

KEGG orthology group: K00016, L-lactate dehydrogenase [EC: 1.1.1.27] (inferred from 78% identity to azl:AZL_026880)

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.1.27)" in subsystem Fermentations: Lactate or Fermentations: Mixed acid (EC 1.1.1.27)

MetaCyc Pathways

• Bifidobacterium shunt (15/15 steps found)
• homolactic fermentation (11/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• heterolactic fermentation (14/18 steps found)
• superpathway of glucose and xylose degradation (13/17 steps found)
• pyruvate fermentation to (S)-lactate (1/1 steps found)
• pyruvate fermentation to acetate and lactate II (3/4 steps found)
• methylglyoxal degradation V (2/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (9/13 steps found)
• L-histidine degradation V (1/2 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (3/8 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.27

Sequence Analysis Tools

See G8AFF4 at UniProt or InterPro

Protein Sequence (313 amino acids)

>AZOBR_RS01310 lactate dehydrogenase (Azospirillum brasilense Sp245)
MKVGIVGAGAVGATAGFAMVMSGAASEVVLVDMNEKLAAAQAQDIAHAVPFARAAQVRHG
GYAALAGAGVVVLAAGVAQRPGETRLQLLERNAAVFGAIIPAVLAAAPDAILLVATNPLD
VMTQIATRISGLPPSRVIGSGTILDTARFRALLADRLGVTPKSVHAHVVGEHGDSEVLLW
SGATVACLPVDRGAERLKRPLTAEDRAAIDEGVRRAAYHIIDGKGHTAFGIGGGLSRIVA
AIAGDERAVLTCSILNDEVLGVPDVALSLPRVIGAGGVLETVMPDLSEEEAAALKRSAEI
LKEAVTSVEKSLP