Protein Info for AZOBR_RS00075 in Azospirillum brasilense Sp245

Annotation: glycerol-3-phosphate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01547: SBP_bac_1" amino acids 43 to 343 (301 residues), 98.2 bits, see alignment E=9.3e-32 PF13416: SBP_bac_8" amino acids 48 to 358 (311 residues), 110.6 bits, see alignment E=1.2e-35

Best Hits

Swiss-Prot: 45% identical to UGPB_YERPS: sn-glycerol-3-phosphate-binding periplasmic protein UgpB (ugpB) from Yersinia pseudotuberculosis serotype I (strain IP32953)

KEGG orthology group: K05813, sn-glycerol 3-phosphate transport system substrate-binding protein (inferred from 76% identity to azl:AZL_006680)

MetaCyc: 46% identical to sn-glycerol 3-phosphate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-34-RXN [EC: 7.6.2.10]; 7.6.2.10 [EC: 7.6.2.10]

Predicted SEED Role

"Glycerol-3-phosphate ABC transporter, periplasmic glycerol-3-phosphate-binding protein (TC 3.A.1.1.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization (TC 3.A.1.1.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AE60 at UniProt or InterPro

Protein Sequence (445 amino acids)

>AZOBR_RS00075 glycerol-3-phosphate ABC transporter substrate-binding protein (Azospirillum brasilense Sp245)
MLTRRKLAVSTAAFALAAGFTGAAYAQQKTVVEFWHGLPQPLGGQLEQVVKGFNDSQDKI
QVNPTFKGSYPETMQAAIAAFRAGNAPHVVQMFEVGTATMLSAGPAIKPVHQLMSETGSG
FDAAAYIPAVRGYYSSKDGKMMALPFNSSTTITFYNKDAFQKAGLDPNKPPATWPEVADA
ARKLKAAGVTCPMTFSWPTWTQFEQVGALHDKPFASEANGFGGLNATLKINDPFFVKHLQ
TLIDLQKEGALRYGGRDNAADSLFPSGECAMIQASSGLRGRIIKEAKFNWGAAPLPYWPD
AIASPKNSIIGGAAFWVMTSPKRTADEYKAVAAFFSYLARPEVDAKWHMDTGYVPVTLQG
FELAKKEGFYDKNPGAEVPAEQLTRTATTENTMGLRLGNLPEIRNIIQEEMEKAFQGQQT
AQQALDNAVKRGDTVLRNFERANKN