Protein Info for AO356_30445 in Pseudomonas fluorescens FW300-N2C3

Annotation: antibiotic transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00529: CusB_dom_1" amino acids 36 to 346 (311 residues), 51 bits, see alignment E=2.3e-17 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 38 to 360 (323 residues), 249.4 bits, see alignment E=2.2e-78 PF16576: HlyD_D23" amino acids 61 to 278 (218 residues), 49.1 bits, see alignment E=9.3e-17 PF13533: Biotin_lipoyl_2" amino acids 65 to 110 (46 residues), 26.5 bits, see alignment 8.4e-10 PF13437: HlyD_3" amino acids 161 to 275 (115 residues), 26.2 bits, see alignment E=2.2e-09

Best Hits

Swiss-Prot: 66% identical to MEPA_PSEPU: Multidrug/solvent efflux pump periplasmic linker protein MepA (mepA) from Pseudomonas putida

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a2036)

MetaCyc: 52% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WB78 at UniProt or InterPro

Protein Sequence (382 amino acids)

>AO356_30445 antibiotic transporter (Pseudomonas fluorescens FW300-N2C3)
MPFKPIFPALVTAIAFATMLSGCNKEQAAATPAQAPQVSVVTLKPQAVTLFNDLPGRTSA
YRLAEVRPQVDGIVLKRLFKEGADIKAGQQLYQIDPSTYEVTLASAQASLEQSRSLTTRY
RQLIDEQAVSKQEYDTARSQQMQAEAQVKGAQINLRYTKMFAPISGRIGRSAVTEGALVS
NGQAAPLAIIQQLDPIYVDVTQPYGEVLKLRRALESGQLQKTADRAAKVSLTLQDGSQYP
LQGSLEFSEVSVDEATGSVTLRAVFPNPDHLLLPGIFVHARLQEGVAEQAILAPQIGVTR
DLKGTPTAMVVTADNKVEQRQLKVGRTVGANVLVESGLSAGDKIIVEGLQFIKPGITVVA
QEAMTDEANRAPVALNTSAKAE