Protein Info for AO356_28540 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): D-mannose transporter
Rationale: Specific phenotype on D-mannose as the carbon source. No other transporter was apparent in the fitness data.
Original annotation: hexuronate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details transmembrane" amino acids 46 to 66 (21 residues), see Phobius details amino acids 76 to 106 (31 residues), see Phobius details amino acids 159 to 180 (22 residues), see Phobius details amino acids 221 to 242 (22 residues), see Phobius details amino acids 262 to 283 (22 residues), see Phobius details amino acids 295 to 314 (20 residues), see Phobius details amino acids 320 to 341 (22 residues), see Phobius details amino acids 353 to 377 (25 residues), see Phobius details amino acids 383 to 405 (23 residues), see Phobius details PF07690: MFS_1" amino acids 13 to 363 (351 residues), 184.7 bits, see alignment E=3.6e-58 amino acids 291 to 415 (125 residues), 40.9 bits, see alignment E=1.9e-14 PF00083: Sugar_tr" amino acids 38 to 180 (143 residues), 29.9 bits, see alignment E=4.3e-11

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a2373)

Predicted SEED Role

"Hexuronate transporter" in subsystem Alginate metabolism or D-Galacturonate and D-Glucuronate Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WPT3 at UniProt or InterPro

Protein Sequence (430 amino acids)

>AO356_28540 D-mannose transporter (Pseudomonas fluorescens FW300-N2C3)
MFGQGRSLIIIMLFLAGVINYLDRSALSVAAPFIQKDYGLSTGEMGMIFSSFFVGYAAFN
FIGGWAADRYGAKTTLLLAMVLWSLFSGLTVLTVGFASLVLIRILFGMGEGPLSVTTSKM
VNNWYTPKRRARAIGASMSGTPLGGAISGPVVGFIAVTYGWKISFIIIMLIGLVWAAVWF
KFVKERPEGEGAEDILRAEGQGELAAQPVFPLRFYLKQPTVLFTSLAFFSYNYTLFFFLT
WFPSYLTMAHGLNVKDMSIATVIPWVLGFLGLALGGFISDFVFKKTGRMMFSRKVVLVTC
LLACAVCIACAGMVTTLYPAVILVALAVFFLYLTGAIYWAIIQDTVPAARVGGVSGFMHF
LANTSGIVGPTLTGFLVQFTGSFTSAFLLAGLLTVIGAVCVARYVKPLSVADTGNAAAQS
PQPVSALGRS