Protein Info for AO356_28500 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): Xylose isomerase (EC 5.3.1.5)
Rationale: Specifically important for utilizing D-Xylose. Automated validation from mutant phenotype: the predicted function (XYLISOM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: xylose isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 93% identical to XYLA_PSEPF: Xylose isomerase (xylA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01805, xylose isomerase [EC: 5.3.1.5] (inferred from 96% identity to pba:PSEBR_a2597)

MetaCyc: 68% identical to xylose isomerase (Escherichia coli K-12 substr. MG1655)
Xylose isomerase. [EC: 5.3.1.5]

Predicted SEED Role

"Xylose isomerase (EC 5.3.1.5)" in subsystem Xylose utilization (EC 5.3.1.5)

MetaCyc Pathways

superpathway of glucose and xylose degradation (17/17 steps found)
D-xylose degradation I (2/2 steps found)
superpathway of pentose and pentitol degradation (15/42 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X5P1 at UniProt or InterPro

Protein Sequence (438 amino acids)

>AO356_28500 Xylose isomerase (EC 5.3.1.5) (Pseudomonas fluorescens FW300-N2C3)
MPYFPGVDKIRYEGPASDSPLAFRHYDANRLVLGKPMREHLRMAVCYWHTFVWPGSDVFG
AGTFKRPWQHAGDPMEVAIGKAEAAFEFFSKLGIDYYCFHDTDVAPEGGSLKEYRNHFAQ
MIDHLERHQEQTGIKLLWGTANCFSNPRFAAGAASNPDPEVFACAAAQVFSAMNATQRLK
GANYVLWGGREGYETLLNTDLKHEREQLGRFMRMVVEHKHKIGFKGDLLIEPKPQEPTKH
QYDYDSATVFGFLQQFGLENEIKVNIEANHATLAGHSFHHEIATAVSLGIFGSIDANRGD
PQNGWDTDQFPNSVEEMTLATYEILKAGGFGNGGFNFDSKVRRQSVDDIDLFHGHVAAMD
VLALALERAAAMVQNDQLQQFKDQRYAGWQQPFGKAVLAGDFSLESLAEHAFTNELNPQA
VSGRQEMLENVVNRFIYP