Protein Info for AO356_28055 in Pseudomonas fluorescens FW300-N2C3

Annotation: quinonprotein alcohol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 621 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 33 to 584 (552 residues), 781.3 bits, see alignment E=2.3e-239 PF13570: PQQ_3" amino acids 79 to 119 (41 residues), 18.1 bits, see alignment 4.6e-07 amino acids 120 to 168 (49 residues), 22.6 bits, see alignment 1.8e-08 amino acids 508 to 548 (41 residues), 26 bits, see alignment 1.5e-09 PF13360: PQQ_2" amino acids 94 to 240 (147 residues), 55.4 bits, see alignment E=1.1e-18 amino acids 498 to 566 (69 residues), 39.2 bits, see alignment E=9.9e-14 PF01011: PQQ" amino acids 101 to 127 (27 residues), 25.5 bits, see alignment (E = 1.2e-09) amino acids 150 to 184 (35 residues), 24.8 bits, see alignment (E = 2.1e-09) amino acids 530 to 566 (37 residues), 41.1 bits, see alignment 1.4e-14

Best Hits

Swiss-Prot: 89% identical to QEDH_PSEPU: Quinoprotein alcohol dehydrogenase (cytochrome c) (qedA) from Pseudomonas putida

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a2660)

MetaCyc: 86% identical to alcohol dehydrogenase (cytochrome c550) monomer (Pseudomonas aeruginosa)
RXN-11333 [EC: 1.1.2.8]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WDR9 at UniProt or InterPro

Protein Sequence (621 amino acids)

>AO356_28055 quinonprotein alcohol dehydrogenase (Pseudomonas fluorescens FW300-N2C3)
MRIKTLPALSALTVALLLAGSLSLSPLANAAAPGVSWEDIANDHLTTQDVLQYGMGTNAQ
RWSPLVQVNDKNVFKLTPAWSYSFGDEKQRGQESQAIVRDGVVYVTGSYSRVFALDARTG
KRLWTYNHRLPDNIRPCCDVVNRGAAIYGDKIYFGTLDARVVALDKNTGKVVWNKKFGDH
AGGYTMTGAPVLIKDKTSGKVLLIHGSSGDEFGVVGQLFARDPETGEEVWMRPFVEGHMG
RLNGKDSTPTGDVKAPSWPDDKTTETGKVEAWSHGGGAPWQSASFDAETNTIIVGAGNPG
PWNTWARTAKDGNPHDYDSLYTSGQVGVDPSTGEVKWFYQHTPNDAWDFSGNNELVLFDY
KDKDGKVVKATAHADRNGFFYVVDRNNGKLQNAFPFVDNITWASHIDLKTGRPVENPGQR
PAKPLPGETKGKPVEVSPPFLGGKNWNPMAYSQDTGLFYVPGNQWKEEYWTEEVNYKKGS
AYLGMGFRIKRMYDDHVGSLRAMNPTTGKVVWEHKEALPLWAGVLATKGNLVFTGTGDGF
FKAFDAKTGKELWKFQTGSGIVSPPITWEQDGEQYIGVTVGYGGAVPLWGGDMAELTKPV
AQGGSFWVFKIPSWDKATAQK