Protein Info for AO356_27890 in Pseudomonas fluorescens FW300-N2C3

Annotation: nitrilase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 6 to 277 (272 residues), 166.5 bits, E=3.7e-53

Best Hits

KEGG orthology group: K01501, nitrilase [EC: 3.5.5.1] (inferred from 98% identity to pba:PSEBR_a3130)

Predicted SEED Role

"Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine" (EC 3.5.5.1)

MetaCyc Pathways

• indole-3-acetate biosynthesis V (bacteria and fungi) (1/1 steps found)
• indole glucosinolate activation (herbivore attack) (8/13 steps found)
• indole-3-acetate biosynthesis II (5/12 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Cyanoamino acid metabolism
• Nitrogen metabolism
• Styrene degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.5.1

Sequence Analysis Tools

See A0A0N9X6K5 at UniProt or InterPro

Protein Sequence (307 amino acids)

>AO356_27890 nitrilase (Pseudomonas fluorescens FW300-N2C3)
MPKSIVAALQIGSLPDGKGETLAQILSYEDAIRQAGARLVVMPEALLGGYPKGEGFGTQL
GYRLPEGREAFARYFANALDVPGVETEALAGLSARTGASLVLGVIERVGSTLYCTALYFE
PDAGLVARHRKLMPTGTERLIWGKGDGSTLPVIDSQVGRVGAAVCWENMMPLLRTAMYAK
GVEVWCAPTVDEREMWQVTMRHIAHEGRCFVVSACQVQASPQALGIDVAHWPVERPLIAG
GSVIVGPMGDVLAGPLKDTAGLLTAEIDTDDLVRARYDYDVVGHYARPDVFELVVDERAK
PGVRFTA