Protein Info for AO356_27190 in Pseudomonas fluorescens FW300-N2C3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 transmembrane" amino acids 233 to 244 (12 residues), see Phobius details PF05368: NmrA" amino acids 2 to 114 (113 residues), 36 bits, see alignment E=2.7e-12 PF04321: RmlD_sub_bind" amino acids 2 to 114 (113 residues), 29.3 bits, see alignment E=2.4e-10 PF01370: Epimerase" amino acids 2 to 224 (223 residues), 91.6 bits, see alignment E=2.8e-29 PF00106: adh_short" amino acids 2 to 117 (116 residues), 26.5 bits, see alignment E=2.1e-09 PF02719: Polysacc_synt_2" amino acids 2 to 118 (117 residues), 34.9 bits, see alignment E=5e-12 PF16363: GDP_Man_Dehyd" amino acids 3 to 154 (152 residues), 47.6 bits, see alignment E=8.7e-16 PF01073: 3Beta_HSD" amino acids 3 to 152 (150 residues), 72.7 bits, see alignment E=1.3e-23 PF07993: NAD_binding_4" amino acids 4 to 180 (177 residues), 50.9 bits, see alignment E=6.1e-17 PF13460: NAD_binding_10" amino acids 6 to 169 (164 residues), 64.7 bits, see alignment E=5.1e-21

Best Hits

Predicted SEED Role

"Dihydroflavonol-4-reductase (EC 1.1.1.219)" in subsystem Biflavanoid biosynthesis or Tannin biosynthesis (EC 1.1.1.219)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.219

Use Curated BLAST to search for 1.1.1.219

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WP92 at UniProt or InterPro

Protein Sequence (323 amino acids)

>AO356_27190 hypothetical protein (Pseudomonas fluorescens FW300-N2C3)
MILVTGASGCLGSHIVRELLEQGENVAVLVLPNDPAAALQSLREGLVWREGDLLDPVSLG
SALEGVTQIYHAAGLALPYECERKRMFAVNVQGTVNLLRAARDAGVERILHVSSIAAVGY
PAEEADESMIYNGDAFRLAYMHSKRDAEDQVRSFCASGMDIVIACPSAVIASNCDVLHGW
GKVMLDLQLRRLPFIPPGGIAVIGAQDLVRGLLAVMAKGKSGERYILNAHNVSYLELFTC
MAAAMAVEAPRWQPRTLGWIVALLRPLDVLLTRIPLRLSADVVALLGRQVHYRSDKARRQ
LGFAPTQSLEHMVHEAASWLTSR