Protein Info for AO356_26995 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): DNA damage response nuclease
Rationale: Conserved and specific phenotype: important for resisting cisplatin. Contains a VRR-NUC domain that is predicted to have nuclease activity.
Original annotation: nuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 transmembrane" amino acids 384 to 396 (13 residues), see Phobius details PF21315: FAN1_HTH" amino acids 10 to 91 (82 residues), 125 bits, see alignment E=1.3e-40 PF18081: FANC_SAP" amino acids 100 to 145 (46 residues), 38.2 bits, see alignment 2e-13 PF08774: VRR_NUC" amino acids 433 to 545 (113 residues), 129.6 bits, see alignment E=8.9e-42

Best Hits

KEGG orthology group: None (inferred from 92% identity to pba:PSEBR_a2829)

Predicted SEED Role

"Hypothetical protein, restriction endonuclease-like VRR-NUC domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WP60 at UniProt or InterPro

Protein Sequence (576 amino acids)

>AO356_26995 DNA damage response nuclease (Pseudomonas fluorescens FW300-N2C3)
VATRSLDDPYYYLNNFQQVLAWLSQRYADVLSHEEQRFIDDFAALPRASQGLLVRMVMRK
GLRFRHSKLSYPEIGDIGAAVAPLLALGWVEEQAPIGLVELFDVLLKAEILLCLGHLIEH
PKAKKTEWLQALDNHPSPLQPFRTWCPQLDERLYSLTIMDLCDRLRLMFFGNLYQDWSEF
VLADLGIFTYETVEFSAESRGLRSREDVDACLFLHDCQQRFEAGEPLEDIVAQVNELSLE
NPWLERRRGKLLFQIGQHGERIGDFALALCIYRDCTYPGARLRMVRVLERIGEYALALEL
GEAAAQTPQSAAETQALLRIVPRLRRKLGGPPVPRVMAREVERLELHLPRVDPGLSVEYH
VQAHLHDETAPVHYVENSLINSLFGLLCWPAIFAPLPGAFFHPFQRGPVDLLNEDFHARR
AELFAACLAELDDGRYRQTIGRRYVEKWGVQSPFVFWGALSEPLLEQALDCLPAEHLKHW
FQRLLLDIKANRAGMPDLIQFWPQHKTYRMIEVKGPGDRLQDNQLRWLEFCHEHQMPVAV
CYVQWAASAIASELAPAEDEPPQIIYGSELARDGRT